Department of Biochemistry and Molecular Biology, University of Szeged, Szeged, Hungary.
FEBS Lett. 2012 Sep 21;586(19):3279-86. doi: 10.1016/j.febslet.2012.06.051. Epub 2012 Jul 13.
ADA2 adaptor proteins are essential subunits of GCN5-containing histone acetyltransferase (HAT) complexes. In metazoa ADA2a is present in the histone H4-specific ATAC, and ADA2b in the histone H3-specific SAGA complex. Using domain-swapped ADA2 chimeras, we determined that the in vivo function of Drosophila melanogaster SAGA and ATAC HAT complexes depend on the C-terminal region of the ADA2 subunit they contain. Our findings demonstrate that the ADA2 C-terminal regions play an important role in the specific incorporation of ADA2 into SAGA- or ATAC-type complexes, which in turn determines H3- or H4-specific histone targeting.
ADA2 衔接蛋白是 GCN5 包含的组蛋白乙酰转移酶 (HAT) 复合物的必需亚基。在后生动物中，ADA2a 存在于组蛋白 H4 特异性的 ATAC 中，ADA2b 存在于组蛋白 H3 特异性的 SAGA 复合物中。使用结构域交换的 ADA2 嵌合体，我们确定了果蝇 SAGA 和 ATAC HAT 复合物的体内功能取决于它们所包含的 ADA2 亚基的 C 末端区域。我们的研究结果表明，ADA2 C 末端区域在 ADA2 特异性掺入 SAGA 或 ATAC 型复合物中起着重要作用，这反过来又决定了 H3 或 H4 特异性组蛋白的靶向。
