7-氨基放线菌素 D 用于流式细胞术的细胞凋亡染色。

7-Aminoactinomycin D for apoptosis staining in flow cytometry.

机构信息

Department of Clinical Pharmacology and Pharmacoepidemiology, University of Heidelberg, 69120 Heidelberg, Germany.

出版信息

Anal Biochem. 2012 Oct 1;429(1):79-81. doi: 10.1016/j.ab.2012.07.005. Epub 2012 Jul 14.

DOI:10.1016/j.ab.2012.07.005

Abstract

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared the results obtained by staining with 1, 5, 10, and 20μg/ml 7-AAD for 20min with the PE-Annexin V Apoptosis Detection Kit and Cell Death Detection ELISA(PLUS) in lymphocytes and CEM human leukemia cells. The results showed that 7-AAD staining with 5, 10, and 20μg/ml, but not with 1μg/ml, is suitable for quantification of apoptosis in flow cytometry.

摘要

7-氨基放线菌素 D（7-AAD）是一种 DNA 染料，可在流式细胞术中区分活细胞、凋亡细胞和晚期凋亡/死亡细胞。已经描述了几种使用 7-AAD 的染色方案，但关于 7-AAD 浓度对读数影响的数据尚不可用。因此，我们比较了用 1μg/ml、5μg/ml、10μg/ml 和 20μg/ml 的 7-AAD 染色 20min 与 PE-Annexin V 凋亡检测试剂盒和 CEM 人白血病细胞中的细胞死亡检测 ELISA(PLUS)在淋巴细胞中的结果。结果表明，5μg/ml、10μg/ml 和 20μg/ml 的 7-AAD 染色，但 1μg/ml 的 7-AAD 染色不适合流式细胞术定量检测凋亡。