Pfister H, Gross G, Hagedorn M
J Invest Dermatol. 1979 Nov;73(5):349-53. doi: 10.1111/1523-1747.ep12550387.
Multiple flat wart-like lesions of a renal allograft recipient were shown to contain HPV 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against HPV [1--5]. The patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). Papillomaviruses were isolated and after in vitro iodination 3 major proteins (MW 70.000, 56.000 and 43.000) were detected by SDS polyacryalmide gel electrophoresis. DNA was extracted from the warts and cleaved with the restriction endonuclease Hae III. Distinct bands were discernible within the background of cellular DNA and these fragments were identified as papillomavirus DNA by blot hypbridization with 32P-labeled viral DNA.
通过用针对人乳头瘤病毒（HPV）的单特异性动物抗血清进行间接免疫荧光检测，发现一名肾移植受者的多个扁平疣状病变含有HPV 3或一种血清学交叉反应病毒[1 - 5]。患者血清显示出病毒特异性抗体（免疫荧光滴度为1/80）。分离出乳头瘤病毒，经体外碘化后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳检测到3种主要蛋白质（分子量分别为70,000、56,000和43,000）。从疣中提取DNA，并用限制性内切酶Hae III切割。在细胞DNA背景中可辨别出不同的条带，通过与32P标记的病毒DNA进行印迹杂交，这些片段被鉴定为乳头瘤病毒DNA。
