Department of Physical Chemistry-Interfacial Phenomena, Faculty of Chemistry, Maria Curie-Sklodowska University, Lublin, Poland.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:44-8. doi: 10.1016/j.colsurfb.2012.06.013. Epub 2012 Jun 23.
DPPC/Cholesterol liposomes of average diameter below 100nm were used as a matrix for calcium carbonate precipitation. Adsorption of calcium ions on the vesicles was determined via zeta potential measurement. It was found that with increasing calcium ions concentration the electrokinetic potential of the vesicles varied toward more positive values. The changes became smaller with the cholesterol content increase. Accumulation of calcium ions close to the vesicles membranes lead to attraction of CO(3)(2-) ions and enhances nucleation and growth of small calcium carbonate crystals that aggregates within lipid vesicles forming porous balls aggregates. However, dipalmitoylphosphatidylcholine (DPPC) does not change the CaCO(3) crystal forms and calcite is the only form obtained during precipitation. Moreover, the influence of the phospholipid on the calcium carbonate precipitation is enhanced by the induction of cholesterol to the lipid membranes.
平均直径低于 100nm 的 DPPC/胆固醇脂质体被用作碳酸钙沉淀的基质。通过测量zeta 电位来确定钙离子在囊泡上的吸附。结果发现，随着钙离子浓度的增加，囊泡的动电势向更正值变化。随着胆固醇含量的增加，这种变化变得更小。钙离子在靠近囊泡膜的地方聚集，导致 CO(3)(2-)离子的吸引，并增强小碳酸钙晶体的成核和生长，这些晶体在脂质囊泡内聚集形成多孔球状聚集物。然而，二棕榈酰磷脂酰胆碱（DPPC）不会改变碳酸钙晶体的形态，在沉淀过程中仅得到方解石。此外，通过向脂质膜中诱导胆固醇，可以增强磷脂对碳酸钙沉淀的影响。
