Department of Physical Chemistry, Faculty of Chemistry University of Sofia, Sofia, Bulgaria.
Colloids Surf B Biointerfaces. 2013 Jan 1;101:135-42. doi: 10.1016/j.colsurfb.2012.05.038. Epub 2012 Jun 15.
It is now established that the surface activity of the clinically used lung surfactant is reduced by serum proteins and can be restored by adding the hydrophilic polymers. The mechanisms of lung surfactant inactivation by serum proteins and restoring effect by the hydrophilic polymers remain not completely understood. In this paper the state and rheological dilatational properties of surface films formed from clinical lung surfactant Exosurf, Survanta, Curosurf and Alveofact in the presence of serum albumin (BSA) and hydrophilic polymers polyvinylpyrrolidone (PVP), polyethylene glycol (PEG) and Dextran were studied. The obtained results suggest that the lung surfactant and BSA mixtures spread at air-water interface form a DPPC/BSA mixed monolayers with lower content of DPPC. The presence of hydrophilic polymers PVP, PEG and Dextran restore the DPPC content in the surface film. The effectiveness of the DPPC spreading and formation of better compacted film increases in order Exosurf, Survanta, Curosurf, Alveofact. The obtained results are in accordance with the generally admitted ideas about the mechanisms of serum protein inactivation and restoring effect of hydrophilic polymers based on the previously studies of the lung surfactant adsorption rate.
现在已经确定，临床上使用的肺表面活性剂的表面活性会被血清蛋白降低，而通过添加亲水性聚合物可以使其恢复。然而，血清蛋白使肺表面活性剂失活的机制以及亲水性聚合物的恢复作用仍不完全清楚。本文研究了在血清白蛋白（BSA）和亲水性聚合物聚乙烯吡咯烷酮（PVP）、聚乙二醇（PEG）和葡聚糖存在的情况下，临床用肺表面活性剂 Exosurf、Survanta、Curosurf 和 Alveofact 形成的表面膜的状态和流变扩张特性。结果表明，在气液界面上扩散的肺表面活性剂和 BSA 混合物形成 DPPC/BSA 混合单层，其中 DPPC 的含量较低。亲水性聚合物 PVP、PEG 和葡聚糖的存在恢复了表面膜中的 DPPC 含量。DPPC 铺展和形成更紧密膜的有效性按 Exosurf、Survanta、Curosurf、Alveofact 的顺序增加。这些结果与基于先前对肺表面活性剂吸附速率的研究提出的关于血清蛋白失活和亲水性聚合物恢复作用机制的普遍观点一致。
