Department of Global Agricultural Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Fish Shellfish Immunol. 2012 Sep;33(3):597-604. doi: 10.1016/j.fsi.2012.06.034. Epub 2012 Jul 14.
To investigate the effects of dietary β-glucan (0.5 or 1 g kg⁻¹ diet: 0.5-BG, 1-BG) and rutin (0.5 or 1 g kg⁻¹ diet: 0.5-RT, 1-RT) after 10 days in the absence of pathogen challenge on the immune response of Fenneropenaeus chinensis, we determined total hemocyte count (THC) and the expression of four immune-related genes in hemocytes: those for prophenoloxidase (proPO), peroxinectin (PX), lipopolysaccharide and/or β-glucan binding protein (LGBP), and c-type lectin (CL). As a prerequisite for subsequent experiments, cDNA encoding proPO of the fleshy shrimp, Fenneropenaeus chinensis (f-proPO) was obtained from hemocytes; it had a full length of 3023 bp, with an open reading frame (ORF) of 2061bp, a 105-bp 5'-untranslated region, and a 906-bp 3'-untranslated region containing the poly A signal. The THCs of shrimp fed β-glucan of 1 g kg⁻¹ diet, and rutin of 1 g kg⁻¹ diet were significantly higher than that of the control (P < 0.05). The expression of proPO mRNA was slightly downregulated and that of LGBP mRNA was upregulated (except in 1-RT). PX and CL mRNA remained constitutively expressed in all groups. Our results reveal that β-glucan and rutin dietary supplements have minimal effect on immune response in the absence of pathogen challenge.
为了研究在没有病原体挑战的情况下，连续 10 天补充饲料 β-葡聚糖（0.5 或 1 g/kg 饲料：0.5-BG，1-BG）和芦丁（0.5 或 1 g/kg 饲料：0.5-RT，1-RT）对中国明对虾免疫反应的影响，我们测定了总血细胞计数（THC）和血细胞中 4 种免疫相关基因的表达：酚氧化酶原（proPO）、过氧化物酶（PX）、脂多糖和/或β-葡聚糖结合蛋白（LGBP）和 C 型凝集素（CL）。作为后续实验的前提，我们从血细胞中获得了肉质虾中国明对虾（f-proPO）的 proPO 基因的 cDNA；它全长 3023bp，开放阅读框（ORF）为 2061bp，5'-非翻译区 105bp，3'-非翻译区 906bp 包含 poly A 信号。饲喂 1 g/kg 饲料 β-葡聚糖和 1 g/kg 饲料芦丁的虾的 THC 明显高于对照组（P<0.05）。proPO mRNA 的表达略有下调，LGBP mRNA 的表达上调（除 1-RT 外）。PX 和 CL mRNA 在所有组中均保持组成型表达。我们的结果表明，在没有病原体挑战的情况下，β-葡聚糖和芦丁饲料补充剂对免疫反应的影响很小。
