Toxicology Unit, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s/n, 10003 Caceres, Spain.
Toxicology Unit, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s/n, 10003 Caceres, Spain.
Environ Toxicol Pharmacol. 2012 Sep;34(2):466-472. doi: 10.1016/j.etap.2012.06.009. Epub 2012 Jul 1.
In the present study the effect of a chronic exposition to the organophosphorous pesticide diazinon on the porphyrin profile in feces of rabbit was evaluated, in order to validate the use of such molecules as non-destructive biomarkers for monitoring exposure of mammals to this environmental xenobiotic. Male and female (12:12) rabbits were exposed to oral single doses of diazinon, feces being sampled at every 10 days, till the end of the experience (30 days). HPLC method was validated from the results obtained, for detection of porphyrins in feces of mammals. Results revealed differences on the porphyrin profile between male and female, the most relevant differences associated to the uroporphyrin levels. In conclusion, porphyrin levels in rabbit's excreta may be used as indicators of exposure to such chemicals, thus providing a useful non-destructive method for monitoring exposure of animals to different environmental pollutants. However, the effect of gender should be taken into account when interpreting results.
本研究旨在评估有机磷农药二嗪农对兔粪便卟啉谱的慢性暴露效应，以期验证此类分子作为监测哺乳动物接触此类环境外来化合物的非破坏性生物标志物的适用性。雄性和雌性（12:12）兔子接受了二嗪农的单次口服剂量，每 10 天采集一次粪便，直至实验结束（30 天）。从获得的结果中验证了 HPLC 方法在检测哺乳动物粪便中卟啉的适用性。结果显示，雄性和雌性之间的卟啉谱存在差异，最相关的差异与尿卟啉水平有关。总之，兔子排泄物中的卟啉水平可以作为接触此类化学物质的指标，从而为监测动物接触不同环境污染物提供了一种有用的非破坏性方法。然而，在解释结果时应考虑到性别因素的影响。
