Laboratory for Ecotoxicology (LECOTOX), Department of Biology and Ecology, University of Novi Sad Faculty of Sciences, 21000 Novi Sad, Serbia.
Laboratory for Ecotoxicology (LECOTOX), Department of Biology and Ecology, University of Novi Sad Faculty of Sciences, 21000 Novi Sad, Serbia.
Environ Toxicol Pharmacol. 2012 Sep;34(2):495-501. doi: 10.1016/j.etap.2012.06.004. Epub 2012 Jun 29.
Previously, we reported that in vivo applied atrazine from postnatal day 23 to 50 induced strong inhibition of testicular steroidogenesis. Therefore, the aim of the present study was to investigate, in the same experimental model, the oxidative status in androgen-producing testicular interstitial compartment characterized by diminished steroidogenesis. In parallel, we determined activities of antioxidative and cytochrome P450 (CYP) xenobiotic-metabolizing enzymes in liver. To confirm the results on atrazine induced-inhibition of testicular androgenesis, we measured ex vivo production of androgen in Leydig cells. The results revealed decreased activity of antioxidant enzymes, especially glutathione S-transferase (GST), but also glutathione peroxidase (GSH-Px) and catalase (CAT) in testicular interstitial cells, in parallel with strongly diminished ex vivo basal and agonist-stimulated Leydig cell androgenesis. In liver, atrazine increased the activity of GSH-Px, GST, and CYP1A1/2 enzyme, but not lipid peroxidation. These results indicate that atrazine markedly affects both antioxidant status and androgenesis in peripubertal rats.
此前，我们曾报道，从出生后第 23 天到 50 天对体内应用莠去津会强烈抑制睾丸类固醇生成。因此，本研究的目的是在相同的实验模型中，研究以类固醇生成减少为特征的雄激素产生睾丸间质腔中的氧化状态。同时，我们还测定了肝脏中抗氧化和细胞色素 P450（CYP）外源物质代谢酶的活性。为了证实莠去津诱导的睾丸雄激素生成抑制的结果，我们测量了离体培养的睾丸间质细胞中雄激素的产生。结果表明，抗氧化酶的活性降低，特别是谷胱甘肽 S-转移酶（GST），但也包括谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT），同时离体基础和激动剂刺激的睾丸间质细胞雄激素生成也明显减少。在肝脏中，莠去津增加了 GSH-Px、GST 和 CYP1A1/2 酶的活性，但不增加脂质过氧化。这些结果表明，莠去津显著影响青春期前大鼠的抗氧化状态和雄激素生成。
