Laboratory of Nutrition Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, , Higashi-ku,, Fukuoka, 812-8581, Japan.
Cardiovasc Drugs Ther. 2012 Oct;26(5):427-31. doi: 10.1007/s10557-012-6403-3.
Ezetimibe has been shown to inhibit dietary cholesterol absorption in animal models and humans, but studies on lymphatic lipid transport have not yet been performed. Rats subjected to permanent lymph duct cannulation were used to investigate the effects of ezetimibe on lipid transport.
Rats were fed diets with and without ezetimibe (5.0 mg/kg), and their lymph was collected after feeding to quantify lymphatic lipid levels. Total cholesterol content in the intestinal mucosa was also measured.
Rats that consumed ezetimibe had significantly lower lymphatic total cholesterol transport with the reduction of esterified cholesterol transport. According to the calculation based on cholesterol consumption, ezetimibe reduced the total cholesterol lymphatic recovery rate by 54 %. We also determined that ezetimibe significantly reduced the total cholesterol content in the intestinal mucosa.
This is the first direct evidence that ezetimibe inhibits esterified but not free cholesterol lymphatic transport in thoracic duct-cannulated rats. The results also indicate that ezetimibe is not involved in the lymphatic transport of triacylglycerols, phospholipids, or α-tocopherol.
依折麦布已被证明可在动物模型和人体中抑制膳食胆固醇的吸收，但尚未对淋巴脂质转运进行研究。本研究通过对永久性淋巴管插管大鼠进行研究，以探讨依折麦布对脂质转运的影响。
给予大鼠含有或不含有依折麦布（5.0mg/kg）的饮食，在喂养后收集其淋巴液以定量测定淋巴脂质水平。同时还测量了肠黏膜中的总胆固醇含量。
与对照组相比，依折麦布组大鼠的总胆固醇经淋巴管转运明显减少，酯化胆固醇的转运也减少。根据胆固醇摄入量的计算，依折麦布使总胆固醇的淋巴回收率降低了 54%。我们还发现，依折麦布显著降低了肠黏膜中的总胆固醇含量。
这是首项直接证据，表明依折麦布可抑制胸导管插管大鼠中酯化但不抑制游离胆固醇的淋巴转运。研究结果还表明，依折麦布不参与三酰甘油、磷脂或α-生育酚的淋巴转运。
