Singh Tejinder Pal, Singh Harpreet, Singh Hazoor
Gulzar Institute of Engineering & Technology, Ludhiana, Punjab - India.
J Appl Biomater Funct Mater. 2014 Jun 12;12(1):48-56. doi: 10.5301/JABFM.2012.9267.
To characterize two thermal sprayed coatings of different particle-sized hydroxyapatite on some biomedical implant materials.
Characterization of the as-sprayed coatings was performed using x-ray diffractometer (XRD), scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS), and x-ray mapping techniques. Bond strength of the sprayed coatings measured as per ASTM-C633 standard and cell culture studies were performed to access the biocompatibility of the coatings.
The results indicated that, HA coatings of 10 μm particle sized powder were completely amorphous, while that of 30 μm sized powder consisted of crystalline HA with minor tetracalcium phosphate (TTCP) and tricalcium phosphate (TCP) phases. This flame spray HA-A and HA-B coatings exhibited higher bond strength (67.8 and 49.3 MPa respectively) in comparison with other thermal spray HA coating techniques reported in literature. In-vitro biocompatibility studies revealed that HA-B coated specimens had good biocompatibility with a human osteosarcoma cell line KHOSNP (R-970-5).
These observations show that particle size of HA powders have a significant effect on the phase composition, microstructure, roughness and biocompatibility of deposited coatings. HA-B coatings were found to be metallurgically better than the HA-A coating from the point of view of biomedical applications.
对某些生物医学植入材料上两种不同粒径的羟基磷灰石热喷涂涂层进行表征。
使用X射线衍射仪（XRD）、扫描电子显微镜/能谱仪（SEM/EDS）和X射线映射技术对喷涂后的涂层进行表征。按照ASTM-C633标准测量喷涂涂层的结合强度，并进行细胞培养研究以评估涂层的生物相容性。
结果表明，粒径为10μm的粉末制成的HA涂层完全无定形，而粒径为30μm的粉末制成的涂层由结晶HA以及少量的磷酸四钙（TTCP）和磷酸三钙（TCP）相组成。与文献中报道的其他热喷涂HA涂层技术相比，这种火焰喷涂的HA-A和HA-B涂层表现出更高的结合强度（分别为67.8和49.3MPa）。体外生物相容性研究表明，HA-B涂层标本与人类骨肉瘤细胞系KHOSNP（R-970-5）具有良好的生物相容性。
这些观察结果表明，HA粉末的粒径对沉积涂层的相组成、微观结构、粗糙度和生物相容性有显著影响。从生物医学应用的角度来看，发现HA-B涂层在冶金方面优于HA-A涂层。
