变形菌转座子突变体和 pckA 突变体的分析。
Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.
机构信息
Department of Marine Biotechnology, Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, Maryland, USA.
出版信息
Appl Environ Microbiol. 2012 Oct;78(19):7120-3. doi: 10.1128/AEM.01794-12. Epub 2012 Jul 13.
Abstract
A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.
摘要
使用 EZ-Tn5 转座酶生成的 Planctomyces limnophilus 突变体被发现其 pckA 内发生插入，该基因编码磷酸烯醇丙酮酸羧激酶。pckA 表达的破坏和酶活性的消除导致在无葡萄糖培养基中生长不良，表明 pckA 在 P. limnophilus 中具有糖异生作用。
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Appl Environ Microbiol. 2011 Aug 15;77(16):5826-9. doi: 10.1128/AEM.05132-11. Epub 2011 Jul 1.
2
Complete genome sequence of Planctomyces limnophilus type strain (Mü 290).嗜湖扁平菌模式菌株（Mü 290）的全基因组序列
Stand Genomic Sci. 2010 Jul 29;3(1):47-56. doi: 10.4056/sigs.1052813.
3
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PLoS Biol. 2010 Jan 19;8(1):e1000281. doi: 10.1371/journal.pbio.1000281.
4
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6
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7
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8
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9
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Annu Rev Microbiol. 2005;59:299-328. doi: 10.1146/annurev.micro.59.030804.121258.
10
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Appl Environ Microbiol. 2004 May;70(5):2816-22. doi: 10.1128/AEM.70.5.2816-2822.2004.
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