Höhn E M, Steigerwald R, Henrich H A
Chirurgische Universitätsklinik, Abt. Experimentelle Chirurgie, Würzburg, F.R.G.
Int J Microcirc Clin Exp. 1990 Nov;9(4):411-22.
The aim of the studies has been to develop an in vitro model which is suitable to study endothelial cell function, especially transendothelial transport phenomena under flow conditions. Microporous glass capillaries, inserted into specially designed perfusion chambers, served as carriers for endothelial cells (EC). A method is described, how to prepare and seed the capillaries in order to have them completely endothelialized. The capillaries were perfused for up to 6 days with 0.2-2.0 ml culture medium/min and wall shear stress was calculated as 0.05-0.5 N/m2. The cells inside the capillary lumen have been observed by phase contrast microscopy and photographed during flow. Our results show that both, vessel geometry and low flow influence the shape and the orientation of EC in vitro. Our model offers a new facility to observe and study a variety of EC functions during flow and under simultaneous microscopic observation.
这些研究的目的是开发一种体外模型，该模型适合于研究内皮细胞功能，特别是在流动条件下的跨内皮运输现象。插入专门设计的灌注室中的微孔玻璃毛细管用作内皮细胞（EC）的载体。本文描述了一种制备毛细管并接种细胞以使它们完全内皮化的方法。毛细管以0.2-2.0 ml/分钟的速度灌注培养基长达6天，壁面剪应力计算为0.05-0.5 N/m²。通过相差显微镜观察毛细管腔内的细胞，并在流动过程中拍照。我们的结果表明，血管几何形状和低流量都会影响体外EC的形状和取向。我们的模型提供了一种新的工具，可在流动过程中并在同步显微镜观察下观察和研究各种EC功能。
