Sui Xing-Ling, Wang Hao, Sun Xiu-Wei
Department of Gastroenterology, the Third Affiliated Hospital of Harbin Medical University, Harbin, China.
Asian Pac J Cancer Prev. 2012;13(4):1389-93. doi: 10.7314/apjcp.2012.13.4.1389.
To discuss the significance of DOG1, CD117 and PDGFRA in the diagnosis of gastrointestinal stromal tumors (GISTs), and analyze their correlations with clinicopathological features and risk ranking.
DOG1, CD117 and PDGFRA were detected with IHC Envision ldpe-g-nvp in 63 GISTs and 43 cases of non-GISTs, and analyzed for relations with clinicopathological factors (gender, age, location, tumor size, mitotic phase, histology) and risk degree.
The positive expression rate of DOG1, CD117 and PDGFRA in GISTs was 84.1% (53/63), 90.5% (57/63), 53.2% (33/63), respectively. Among the 6 CD117 negative cases, all were DOG1 positive and 5 were PDGFRA positive. Rates in patients with non-GISTs was 11.6%, 16.3%, 6.98%, respectively. Expression of DOG1 and PDGFRA demonstrated no significant variation with gender, age, position, tumor size, mitotic phase, histology, and risk rank. However, CD117 was related with position and histology (P=0.008 and P=0.045), those in the mesentery having a higher positive rate than those derived from stomach, small intestine, colon and rectum (50.0% vs 94.7%, P=0.008). Furthermore CD117 was also highly expressed in spindle and epithele types.
DOG1 had a good sensitivity and specificity as a kind of newly discovered marker, especially for KIT negative GISTs. However, DOG1, CD117 and PDGFRA cannot be used for assessing the rish of patients.
探讨DOG1、CD117和血小板衍生生长因子受体α（PDGFRA）在胃肠道间质瘤（GIST）诊断中的意义，并分析它们与临床病理特征及危险度分级的相关性。
采用免疫组织化学Envision法检测63例GIST和43例非GIST中DOG1、CD117和PDGFRA的表达，并分析其与临床病理因素（性别、年龄、部位、肿瘤大小、核分裂象、组织学类型）及危险度的关系。
GIST中DOG1、CD117和PDGFRA的阳性表达率分别为84.1%（53/63）、90.5%（57/63）、53.2%（33/63）。在6例CD117阴性病例中，DOG1均为阳性，5例PDGFRA为阳性。非GIST患者中三者的阳性表达率分别为11.6%、16.3%、6.98%。DOG1和PDGFRA的表达在性别、年龄、部位、肿瘤大小、核分裂象、组织学类型及危险度分级方面差异均无统计学意义。然而，CD117的表达与部位和组织学类型有关（P=0.008，P=0.045），肠系膜来源者阳性率高于胃、小肠、结肠和直肠来源者（50.0% 比94.7%，P=0.008）。此外，CD117在梭形细胞型和上皮样型中也高表达。
DOG1作为一种新发现的标志物，具有较好的敏感性和特异性，尤其对于KIT阴性的GIST。然而，DOG1、CD117和PDGFRA均不能用于评估患者的危险度。
