Li Hong-Li, Huang Ding-Zhi, Deng Ting, Zhou Li-Kun, Wang Xia, Bai Ming, Ba Yi
Department of Gastrointestinal Oncology, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
Asian Pac J Cancer Prev. 2012;13(4):1425-30. doi: 10.7314/apjcp.2012.13.4.1425.
Cyclin L2 is a novel member of the cyclin family, recently implicated in the regulation of cell cycle progression and/or transcriptional regulation. The present study was undertaken to investigate the effects of overexpression on tumor cell growth and chemosensitivity in human gastric cells in vitro. Cyclin L2 was transfected into human gastric cancer cell line BCG823 and expressed with a mammalian expression vector pcDNA3.1. The effects and mechanisms of cyclin L2 on cell growth, cell cycling and apoptosis were studied. Compared to control vectors, overexpression of cyclin L2 inhibited the growth of BCG823 cells and enhance their chemosensitivity to fluorouracil, docetaxel and cisplatin. The anti-proliferative effects of cyclin L2 could be due to G0/G1 arrest and apoptosis. Cyclin L2 induced G0/G1 arrest and apoptosis involved upregulation of caspase-3 and down regulation Bcl-2 and survivin. The results indicated that overexpression of cyclin L2 protein may promote efficient growth inhibition and enhance chemosensitivity to chemotherapeutic agents in human gastric cancer cells by inducing G0/G1 cell cycle arrest and apoptosis.
细胞周期蛋白L2是细胞周期蛋白家族的一个新成员，最近被认为参与细胞周期进程的调控和/或转录调控。本研究旨在探讨过表达细胞周期蛋白L2对人胃癌细胞体外生长及化疗敏感性的影响。将细胞周期蛋白L2转染到人胃癌细胞系BCG823中，并通过哺乳动物表达载体pcDNA3.1进行表达。研究了细胞周期蛋白L2对细胞生长、细胞周期及凋亡的影响和机制。与对照载体相比，细胞周期蛋白L2的过表达抑制了BCG823细胞的生长，并增强了其对氟尿嘧啶、多西他赛和顺铂的化疗敏感性。细胞周期蛋白L2的抗增殖作用可能归因于G0/G1期阻滞和凋亡。细胞周期蛋白L2诱导G0/G1期阻滞和凋亡涉及半胱天冬酶-3的上调以及Bcl-2和生存素的下调。结果表明，细胞周期蛋白L2蛋白的过表达可能通过诱导G0/G1期细胞周期阻滞和凋亡，促进人胃癌细胞的有效生长抑制并增强其对化疗药物的敏感性。
