XU Ou, LI Xiao-ming, WANG Mao-xin, QU Yong-tao, LU Xiu-ying, SHAN Chun-guang, SUN Qing-jia
Department of Otorhinolaryngology, Hebei Medical University, Shijiazhuang 050017, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Apr;47(4):330-3.
To detect the expression of hypoxia inducible factor 1 alpha (HIF-1α), glucose transporter protein-1 (GLUT-1) and vascular endothelial growth factor (VEGF) in human laryngeal carcinoma tissue, and to study the relationship between hypoxia and HIF-1α, GLUT-1, VEGF in human laryngeal carcinoma Hep-2 cells and to explore the effect of HIF-1α, GLUT-1 and VEGF as endogenous hypoxic markers on laryngeal carcinoma.
The expression levels of HIF-1α, GLUT-1 and VEGF were detected in 35 cases of laryngeal carcinoma by SP immunohistochemical methods and in Hep-2 cells by SP immunocytochemical methods. The relationship between HIF-1α and GLUT-1, VEGF protein expression was analyzed.
Of the 35 cases, 16 cases expressed HIF-1α, 16 cases expressed GLUT-1, 19 cases expressed VEGF. The expression of HIF-1α and VEGF were closely correlated with pathologic grading and lymphnode metastasis. GLUT-1 was correlated with lymphnode metastasis. The expression levels of HIF-1α, GLUT-1 and VEGF in Hep-2 cells under hypoxic condition were higher than those under normoxic condition.
HIF-1α may promote the expression of GLUT-1 and VEGF in laryngeal carcinoma, furthermore promote tumor angiogenesis, invasion, and metastasis of the laryngeal carcinoma.
检测缺氧诱导因子1α（HIF-1α）、葡萄糖转运蛋白1（GLUT-1）和血管内皮生长因子（VEGF）在人喉癌组织中的表达，研究缺氧与人喉癌Hep-2细胞中HIF-1α、GLUT-1、VEGF之间的关系，并探讨HIF-1α、GLUT-1和VEGF作为内源性缺氧标志物对喉癌的影响。
采用SP免疫组化方法检测35例喉癌组织中HIF-1α、GLUT-1和VEGF的表达水平，采用SP免疫细胞化学方法检测Hep-2细胞中三者的表达水平。分析HIF-1α与GLUT-1、VEGF蛋白表达之间的关系。
35例中，16例表达HIF-1α，16例表达GLUT-1，19例表达VEGF。HIF-1α和VEGF的表达与病理分级和淋巴结转移密切相关。GLUT-1与淋巴结转移相关。缺氧条件下Hep-2细胞中HIF-1α、GLUT-1和VEGF的表达水平高于常氧条件下。
HIF-1α可能促进喉癌中GLUT-1和VEGF的表达，进而促进喉癌的肿瘤血管生成、侵袭和转移。
