Vance J E, Russell J C
Lipid and Lipoprotein Group, University of Alberta, Edmonton, Canada.
J Lipid Res. 1990 Aug;31(8):1491-501.
The JCR:LA-corpulent male rat, when homozygous for the cp gene (cp/cp) is hyperlipidemic and prone to atherosclerosis. Both male and female cp/cp rats have markedly elevated serum levels of triacylglycerols and phospholipids [Dolphin, P.J. et al. 1987. Biochim. Biophys. Acta. 919: 140-148]. In the present study, monolayer cultures of hepatocytes were prepared from male and female, corpulent and lean, rats. There was a marked hypersecretion of all very low density lipoprotein (VLDL) lipid and apoprotein components from corpulent-derived cells. The increased secretion most likely accounts for the increased levels of VLDL lipids and apoproteins previously observed in serum. In contrast, there was no difference between the corpulent and lean hepatocytes in their secretion of high density lipoprotein (HDL) lipids and apoproteins. The difference in triacylglycerol secretion between the lean and corpulent cells was sustained even when the cells were cultured for 24, 48, and 72 h prior to the experiment, by which time the hormonal differences between the corpulent and lean animals would have been largely eliminated. The magnitude of the difference in triacyglycerol secretion did not diminish with increasing time in culture. The biochemical basis responsible for the hypersecretion of VLDL has not yet been established. However, preliminary results suggest that there is an inherent difference in glycerolipid metabolism in the two types of hepatocytes.
LA肥胖雄性大鼠在纯合cp基因（cp/cp）时会出现高脂血症并易患动脉粥样硬化。雄性和雌性cp/cp大鼠的血清甘油三酯和磷脂水平均显著升高[多尔芬，P.J.等人，1987年。生物化学与生物物理学报。919:140 - 148]。在本研究中，从雄性和雌性、肥胖和瘦的大鼠中制备了肝细胞单层培养物。肥胖来源的细胞中所有极低密度脂蛋白（VLDL）脂质和载脂蛋白成分均有明显的高分泌。分泌增加很可能是先前在血清中观察到的VLDL脂质和载脂蛋白水平升高的原因。相比之下，肥胖和瘦的肝细胞在高密度脂蛋白（HDL）脂质和载脂蛋白的分泌方面没有差异。即使在实验前将细胞培养24、48和72小时，肥胖和瘦细胞之间甘油三酯分泌的差异仍然存在，此时肥胖和瘦动物之间的激素差异已基本消除。甘油三酯分泌差异的幅度不会随着培养时间的增加而减小。导致VLDL高分泌的生化基础尚未确定。然而，初步结果表明，两种类型的肝细胞在甘油脂质代谢方面存在内在差异。
