Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Environ Sci Pollut Res Int. 2013 Apr;20(4):2220-5. doi: 10.1007/s11356-012-1087-6. Epub 2012 Jul 17.
Polycyclic aromatic hydrocarbons (PAHs) are persistent, bioaccumulative, and toxic chemicals and are listed as priority pollutants by the US EPA. Although they are sparsely soluble in water, their solubility can be increased by binding to dissolved organic matter in natural waters, which will further increase their environmental risk as toxic pollutants. In this study, the interaction between PAHs, exemplified by fluorene and anthracene, and fulvic acid (FA) was studied using fluorescence quenching titration method with fluorescence emission spectra, respectively. The association of FA with the mixture of fluorene and anthracene was also evaluated by excitation-emission matrix (EEM) fluorescence spectrometry combined with parallel factor (PARAFAC) analysis. Results demonstrate that EEM fluorescence spectrometry with PARAFAC analysis was sensitive and reliable to determine the binding properties of PAHs with FA in a mixed aqueous solution. The conditional stability constants and binding capacities show that both PAHs bind to FA tightly.
多环芳烃（PAHs）是持久性、生物累积性和有毒的化学物质，被美国环保署列为优先污染物。尽管它们在水中的溶解度很低，但可以通过与天然水中溶解的有机物结合来增加其溶解度，这将进一步增加它们作为有毒污染物的环境风险。在这项研究中，使用荧光猝灭滴定法和荧光发射光谱分别研究了芴和蒽等多环芳烃与富里酸（FA）的相互作用。还通过激发-发射矩阵（EEM）荧光光谱结合平行因子（PARAFAC）分析评估了 FA 与芴和蒽混合物的缔合。结果表明，EEM 荧光光谱与 PARAFAC 分析相结合，对于确定混合水溶液中 PAHs 与 FA 的结合特性非常敏感和可靠。条件稳定常数和结合容量表明，两种 PAHs 都与 FA 紧密结合。
