State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Poult Sci. 2012 Aug;91(8):1950-5. doi: 10.3382/ps.2011-02114.
Feathers are appendages of skin in avians and provide a model for analyzing skin appendage morphogenesis. The feathers of Chinese Silky (CS) and White Leghorn (WL) chickens have distinct phenotypes. Based on preliminary genetic results, we cloned the chicken gene sobp (encoding sine oculis binding protein homolog, which is expressed at a higher level in the dorsal skin and in the feather follicle in the WL compared with the CS. The reverse-transcription PCR and quantitative real-time PCR indicated that sobp was expressed in many tissues and was continuously expressed during embryonic development in both strains. Northern and Western blotting indicated that the mRNA of sobp was approximately 5 kb and the SOBP protein was approximately 96 kDa. The expressions of signaling molecules that affect feather development were similar. This study is the first report of sobp expression in chickens. Our results suggest that sobp might regulate distinct feather type.
羽毛是禽类皮肤的附属物，为分析皮肤附属物形态发生提供了模型。中国丝羽鸡（CS）和白来航鸡（WL）的羽毛具有明显不同的表型。基于初步的遗传结果，我们克隆了鸡 sobp 基因（编码 sine oculis 结合蛋白同源物，该基因在 WL 中的背部皮肤和羽毛毛囊中的表达水平高于 CS。逆转录 PCR 和定量实时 PCR 表明 sobp 在两种鸡的许多组织中表达，并在胚胎发育过程中持续表达。Northern 和 Western 印迹表明 sobp 的 mRNA 约为 5 kb，SOBP 蛋白约为 96 kDa。影响羽毛发育的信号分子的表达相似。本研究首次报道了 sobp 在鸡中的表达。我们的结果表明，sobp 可能调节不同的羽毛类型。
