Feed Science Institute, Zhejiang University, Zijingang Campus, Hangzhou, 310058, China.
Poult Sci. 2012 Aug;91(8):1974-82. doi: 10.3382/ps.2012-02164.
To better understand the digestive capacity in domestic pigeons (Columba livia), this study was conducted to evaluate nutrient transporters and digestive enzymes gene expression in small intestine and yolk sac membrane (YSM) during pre- and posthatch development. We investigated the oligopeptide transporter Pept1, sodium glucose transporter SGLT1, glucose transporter GLUT2, aminopeptidase-N (APN), and sucrase-isomaltase (SI). Intestine was collected at embryo d 12, 14, and 16, day of hatch, and d 1, 3, 5, 8, and 14 posthatch. The YSM was collected at embryo d 12, 14, 16, and day of hatch. The cDNA fragments for Pept1, SGLT1, GLUT2, APN, and SI were isolated and cloned using reverse-transcription PCR. The sequences data showed that these genes were highly identical to the gene of chicken. The mRNA expression of each gene was assayed using real-time PCR. Expression of intestinal nutrient transporters increased linearly (P<0.001) with age whereas that of APN increased quadratically (P<0.001) and SI increased cubically (P=0.001). Levels of PepT1 mRNA were greatest in the duodenum (P=0.001), GLUT2 and SGLT1 mRNA were greatest in the jejunum (P=0.002) and APN were greatest in the ileum (P=0.004). The YSM expressed all the examined genes. The YSM-expressed genes decreased between embryo d 16 and day of hatch, whereas intestine-expressed genes increased. Our findings provide a comprehensive profile of gene expression patterns of nutrient transporter and digestive enzyme in the small intestine and YSM of pigeons and establish a foundation for future research on the nutrients requirements for young pigeons.
为了更好地了解家鸽（Columba livia）的消化能力，本研究评估了小肠道和卵黄囊膜（YSM）中营养转运体和消化酶基因在孵化前后发育过程中的表达。我们研究了寡肽转运体 Pept1、钠葡萄糖转运体 SGLT1、葡萄糖转运体 GLUT2、氨肽酶-N（APN）和蔗糖酶-异麦芽糖酶（SI）。在胚胎第 12、14 和 16 天、孵化当天以及孵化后第 1、3、5、8 和 14 天收集肠道样本。在胚胎第 12、14、16 天和孵化当天收集卵黄囊膜样本。使用反转录 PCR 从肠道和卵黄囊膜中分离和克隆 Pept1、SGLT1、GLUT2、APN 和 SI 的 cDNA 片段。这些基因的序列数据与鸡的基因高度相似。使用实时 PCR 测定每个基因的 mRNA 表达。肠道营养转运体的表达随年龄呈线性增加（P<0.001），而 APN 的表达呈二次方增加（P<0.001），SI 的表达呈三次方增加（P=0.001）。十二指肠中 PepT1 mRNA 水平最高（P=0.001），空肠中 GLUT2 和 SGLT1 mRNA 水平最高（P=0.002），回肠中 APN 水平最高（P=0.004）。YSM 表达所有检测到的基因。从胚胎第 16 天到孵化当天，YSM 表达的基因减少，而肠道表达的基因增加。我们的研究结果为鸽子小肠道和 YSM 中营养转运体和消化酶基因的表达模式提供了全面的概况，并为未来研究幼鸽的营养需求奠定了基础。
