通过电泳和蛋白质印迹法对新分离的18型艾柯病毒变体进行蛋白质分析。
Protein analysis of newly isolated variants of echovirus type 18 by electrophoresis and western blotting.
作者信息
Hayase Y, Toya K, Tanaka T, Tobita K
机构信息
Department of Virology, Jichi Medical School, Tochigi-Ken, Japan.
出版信息
J Med Virol. 1990 Nov;32(3):143-7. doi: 10.1002/jmv.1890320303.
PMID:2280258
Abstract
The protein patterns of two newly isolated antigenic variants of echovirus type 18 were compared with those of the prototype strain. Electrophoretic mobilities of VP1 and VP2 of the variants differed from those of the prototype strain. The antiserum against the prototype strain reacted to VP0 and VP2 in the Western blotting experiment. In addition, the variants differed from the prototype strain in growth behavior in the cultured cells.
摘要
将新分离出的18型埃可病毒的两种抗原变体的蛋白质模式与原型毒株的蛋白质模式进行了比较。这些变体的VP1和VP2的电泳迁移率与原型毒株不同。在蛋白质印迹实验中，针对原型毒株的抗血清与VP0和VP2发生反应。此外，这些变体在培养细胞中的生长行为也与原型毒株不同。
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