Ivanov A V, Luzyanin B P, Kubatiev A A
Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
Bull Exp Biol Med. 2012 Jan;152(3):289-92. doi: 10.1007/s10517-012-1510-5.
Fraction analysis of homocysteine in biological fluids is important for the diagnosis and studies of cardiovascular, nervous, urological, and other diseases. Measurements of total, free, and reduced homocysteine by mass spectrometry with HPLC by means of its modification with N-ethylmaleimide are proposed. The linearity and detection threshold were 0.025-10 and 0.001 μM, respectively, for reduced homocysteine fraction and 0.2-100 and 0.1 μM, respectively, for the rest fractions. The accuracy and reproducibility of the method were within 12%.
生物体液中同型半胱氨酸的组分分析对于心血管疾病、神经疾病、泌尿系统疾病及其他疾病的诊断和研究具有重要意义。本文提出了通过用N - 乙基马来酰亚胺对其进行修饰，采用高效液相色谱 - 质谱联用技术测定总同型半胱氨酸、游离同型半胱氨酸和还原型同型半胱氨酸的方法。还原型同型半胱氨酸组分的线性范围和检测限分别为0.025 - 10 μM和0.001 μM，其余组分的线性范围和检测限分别为0.2 - 100 μM和0.1 μM。该方法的准确度和重现性在12%以内。
