Cai Cheng-Yi, Ma Wu-Hua, Deng Lian
Department of Anesthesiology, First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Apr;32(4):474-7.
To study the regulatory effect of Ligustrazine Injection (LI) on the cellular immune function in patients undergoing autologous blood transfusion (ABT).
Enrolled were 60 patients scheduled for receiving selective lumbar surgery at the Department of Spinal Orthopedics, First Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine during October 2009 to June 2010. They were equally randomized into two groups, the trial group and the control group. LI was given to patients in the trial group by intravenous dripping at the dose of 2 mg/kg 30 min before autologous blood collection. The LI (at the final concentration of 0.005%) was added in the heparin saline solution and the washing saline for recycle blood. No LI was given to patients in the control group. They received the same treatment of the trial group. The operation time, the amount of blood loss and blood transfusion were recorded. Patients' venous blood samples were collected for determining cytokines including interleukin-2 (IL-2), interleukin-10 (IL-10), interferon-gamma (IFN-gamma) by ELISA and calculating IL-2/IL-10 ratio before surgery (T1), 1 h (T2), 1 day (T3), and 5 days (T4) after ABT.
There was no statistical difference in the amount of blood loss and blood transfusion, the levels of IL-2, IL-10, IFN-gamma, or IL-2/IL-10 at T1 between the two groups (P>0.05). Compared with T1 of the same group, the level of IL-2 decreased at T(2-4), IL-10 increased and IL-2/IL-10 decreased at T(2-3) in the two groups. The level of IFN-gamma decreased at T(2-4), IL-2/IL-10 increased at T4, the level of IL-10 decreased at T4 in the control group (P<0.05, P<0.01). The level of IL-10 decreased at T4 in the trial group with statistical difference (P<0.05, P<0.01). Compared with the control group, the level of IL-2, IFN-gamma, and IL-2/IL-10 at T(2-4) were obviously higher in the trial group. But the IL-10 level was lower in the trial group than in the control group at T(2-4) (P<0.05, P<0.01).
The application of LI in ABT had regulatory effects on the balance of cytokines.
研究川芎嗪注射液（LI）对自体输血（ABT）患者细胞免疫功能的调节作用。
选取2009年10月至2010年6月在广州中医药大学第一附属医院脊柱骨科行择期腰椎手术的60例患者。将其随机分为两组，即试验组和对照组。试验组患者在自体采血前30分钟静脉滴注LI，剂量为2mg/kg。将LI（终浓度为0.005%）加入肝素盐水溶液及回收血洗涤盐水中。对照组患者不给予LI，给予与试验组相同的治疗。记录手术时间、失血量及输血量。术前（T1）、ABT后1小时（T2）、1天（T3）及5天（T4）采集患者静脉血样，采用酶联免疫吸附测定法（ELISA）检测细胞因子白细胞介素-2（IL-2）、白细胞介素-10（IL-10）、干扰素-γ（IFN-γ），并计算IL-2/IL-10比值。
两组患者在T1时失血量、输血量、IL-2、IL-10、IFN-γ水平及IL-2/IL-10比值比较，差异无统计学意义（P>0.05）。与同组T1比较，两组患者在T(2-4)时IL-2水平降低，在T(2-3)时IL-10水平升高、IL-2/IL-10比值降低。对照组在T(2-4)时IFN-γ水平降低，在T4时IL-2/IL-10比值升高、IL-10水平降低（P<0.05，P<0.01）。试验组在T4时IL-10水平降低，差异有统计学意义（P<0.05，P<0.01）。与对照组比较，试验组在T(2-4)时IL-2、IFN-γ及IL-2/IL-10水平明显升高，但试验组在T(2-4)时IL-10水平低于对照组（P<0.05，P<0.01）。
LI应用于ABT对细胞因子平衡具有调节作用。
