Hu Wan-Jun, Ma Dan-Wei, Wang Ya-Nan, Zhang Hong
College of Life Science, Sichuan Normal University, Chengdu 610066, China.
Ying Yong Sheng Tai Xue Bao. 2012 Apr;23(4):1077-82.
Chenopodium ambrosioides is an invasive species, which has strong allelopathic effect on surrounding plants. In this study, the methods of soil culture and filter paper culture were adopted to simulate the eluviation and volatilization of the volatile oil from C. ambrosioides, respectively, and to investigate the allelopathy of the volatile oil on the lipid peroxidation and antioxidant enzyme activities of Vicia faba root tip cells, with the mechanisms of the induced tip cell apoptosis analyzed. At the early stage (24 h) of soil culture and filter paper culture, the superoxide dismutase, peroxidase and catalase activities of the tip cells decreased after an initial increase with the increasing dose of the volatile oil, and the malondialdehyde content of the tip cells increased with the increasing volatile oil dose and treated time. At the midterm (48 h) and later (72 h) stages of soil culture and filter paper culture, a typical DNA ladder strip appeared, suggesting that the volatile oil from C. ambrosioides could induce the apoptosis of the tip cells, and the apoptosis was dose- and time dependent. This study showed that the volatile oil from C. ambrosioides could act on its surrounding plants via eluviation and volatilization, making the lipid peroxidation of acceptor plants aggravated and the antioxidant enzyme activities of the plants inhibited, resulting in the oxidative damage and apoptosis of the plant root tip cells, and accordingly, the inhibition of the plant growth. Under soil culture, the root tip cells of V. faba had higher antioxidant enzyme activities and lesser DNA damage, suggesting that the volatile oil from C. ambrosioides via volatilization had stronger allelopathy on the growth of surrounding plants than via eluviation.
土荆芥是一种入侵物种，对周围植物具有强烈的化感作用。本研究采用土壤培养和滤纸培养方法，分别模拟土荆芥挥发油的淋溶和挥发作用，研究挥发油对蚕豆根尖细胞脂质过氧化和抗氧化酶活性的化感作用，并分析诱导根尖细胞凋亡的机制。在土壤培养和滤纸培养的早期（24 h），根尖细胞的超氧化物歧化酶、过氧化物酶和过氧化氢酶活性随着挥发油剂量的增加先升高后降低，根尖细胞丙二醛含量随着挥发油剂量和处理时间的增加而增加。在土壤培养和滤纸培养的中期（48 h）及后期（72 h），出现典型的DNA梯状条带，表明土荆芥挥发油可诱导根尖细胞凋亡，且凋亡具有剂量和时间依赖性。本研究表明，土荆芥挥发油可通过淋溶和挥发作用作用于周围植物，使受体植物脂质过氧化加剧，植物抗氧化酶活性受到抑制，导致植物根尖细胞发生氧化损伤和凋亡，进而抑制植物生长。在土壤培养条件下，蚕豆根尖细胞具有较高的抗氧化酶活性和较小的DNA损伤，表明土荆芥挥发油通过挥发作用对周围植物生长的化感作用强于淋溶作用。
