Oelofsen M J, Gericke A, Smith M S, van der Linde T C
Department of Virology, Medical Faculty, University of the Orange Free State, Bloemfontein, Republic of South Africa.
J Med Entomol. 1990 Nov;27(6):939-44. doi: 10.1093/jmedent/27.6.939.
The mosquito Culex (Culex) theileri Theobald is probably the major vector of Rift Valley fever (RVF) virus during epizootics on the inland plateau of southern Africa. A cell line was established from the tissues of this mosquito species to facilitate laboratory studies on RVF and other arboviruses. After temperature pretreatment, egg rafts from a laboratory colony were disinfected, mechanically disrupted, and grown in preconditioned Eagles minimum essential medium supplemented with 10% fetal calf serum. The cells were characterized by their protein content, karyology, and their susceptibility to three arboviruses. Although high infectivity titers were obtained, no cytopathic effects were produced by these viruses. The cell line was shown to be free from latent viruses. These results show that this cell line may be employed fruitfully in arbovirus studies.
尖音库蚊（库蚊属）泰勒库蚊可能是非洲南部内陆高原动物疫情期间裂谷热（RVF）病毒的主要传播媒介。从这种蚊子的组织中建立了一个细胞系，以促进对裂谷热和其他虫媒病毒的实验室研究。经过温度预处理后，对实验室种群的卵块进行消毒、机械破碎，并在添加了10%胎牛血清的预处理伊格尔氏最低必需培养基中培养。通过细胞的蛋白质含量、染色体学以及它们对三种虫媒病毒的易感性对细胞进行了表征。尽管获得了高感染性滴度，但这些病毒并未产生细胞病变效应。该细胞系被证明没有潜伏病毒。这些结果表明，该细胞系可有效地用于虫媒病毒研究。
