Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90509, USA.
Transfusion. 2013 Apr;53(4):811-5. doi: 10.1111/j.1537-2995.2012.03793.x. Epub 2012 Jul 13.
Titration is a semiquantitative method used to estimate red blood cell (RBC) alloantibody reactivity. The conventional tube test (CTT) technique is the traditional method for performing titration studies. The gel microcolumn assay (GMA) is also a sensitive method to detect RBC alloantibodies. The aim of this study was to compare a GMA with the CTT technique in the performance of Rh and K alloantibody titration.
Patient serum samples that contained an RBC alloantibody with a singular specificity were identified by routine blood bank workflow. Parallel titration studies were performed on these samples by both the CTT method and a GMA (ID-Micro Typing System anti-IgG gel card, Micro Typing Systems, Inc., an Ortho-Clinical Diagnostics Company).
Forty-eight samples were included, including 11 anti-D, five anti-c, 13 anti-E, one anti-C, three anti-e, and 15 anti-K. Overall, the two methods generated identical results in 21 of 48 samples. For 42 samples (87.5%) the two methods generated results that were within one serial dilution, and for the remaining six samples, results were within two dilutions.
GMA systems may perform comparably to the CTT in titrating alloantibodies to Rh and Kell antigens.
滴定是一种半定量方法，用于估计红细胞 (RBC) 同种异体抗体反应性。传统的试管试验 (CTT) 技术是进行滴定研究的传统方法。凝胶微柱检测 (GMA) 也是一种检测 RBC 同种异体抗体的敏感方法。本研究旨在比较 GMA 与 CTT 技术在 Rh 和 Kell 同种异体抗体滴定中的性能。
通过常规的血库工作流程确定含有单一特异性 RBC 同种异体抗体的患者血清样本。对这些样本同时进行 CTT 方法和 GMA（ID-MicroTyping System anti-IgG 凝胶卡，MicroTyping Systems，Inc.，Ortho-Clinical Diagnostics 公司）的平行滴定研究。
共纳入 48 份样本，包括 11 份抗-D、5 份抗-c、13 份抗-E、1 份抗-C、3 份抗-e 和 15 份抗-K。总体而言，两种方法在 48 份样本中的 21 份中产生了相同的结果。对于 42 份（87.5%）样本，两种方法的结果相差一个稀释度，对于其余 6 份样本，结果相差两个稀释度。
GMA 系统在滴定 Rh 和 Kell 抗原的同种异体抗体方面可能与 CTT 具有可比性。
