Zhu Yan, Li Dun-Gao, Sun Zhao-Gui, Chen Xue-Jin, Jiang Man-Xi
Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Committee, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.
Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Zygote. 2014 May;22(2):182-6. doi: 10.1017/S0967199412000366. Epub 2012 Jul 18.
Summary The goal of this project was to determine whether the originating strain of mouse embryonic stem (ES) cells affects the maintenance of their pluripotency under uniform culture conditions. ES cells from two strains of mice, E14 and C2J, were tested. Both ES cell lines were cultured in KOSR + 2i medium and then injected into C57BL/6J blastocysts. Our results demonstrate that this medium could support both E14 and C2J ES cells to keep their pluripotency, though E14 ES cells were found to have a higher chimeric rate than C2J ES cells. However, analysis by backcrossing revealed that C2J and E14 ES cells have the same ability for germline transmission. Our results demonstrate that ES cells derived from E14 and C2J cells have the same capacity for germline transmission when injected into C57BL/6J blastocysts; however, due to the limitation of mixed genetic background between E14 cells and host C57BL/6J embryos, C2J ES cells are preferable to E14 ES cells for use in gene-targeting and should become the cell line of choice for the generation of genetically engineered mutant mouse lines.
摘要 本项目的目标是确定小鼠胚胎干细胞（ES细胞）的原始菌株在统一培养条件下是否会影响其多能性的维持。对来自两种小鼠品系E14和C2J的ES细胞进行了测试。两种ES细胞系均在KOSR + 2i培养基中培养，然后注射到C57BL/6J囊胚中。我们的结果表明，这种培养基可以支持E14和C2J ES细胞保持其多能性，尽管发现E14 ES细胞的嵌合率高于C2J ES细胞。然而，通过回交分析发现，C2J和E14 ES细胞具有相同的种系传递能力。我们的结果表明，当注射到C57BL/6J囊胚中时，源自E14和C2J细胞的ES细胞具有相同的种系传递能力；然而，由于E14细胞与宿主C57BL/6J胚胎之间混合遗传背景的限制，在基因靶向中C2J ES细胞比E14 ES细胞更可取，并且应该成为生成基因工程突变小鼠品系的首选细胞系。
