Klein C, Troedsson M H T
Department of Veterinary Science, Gluck Equine Research Center, Lexington, KY 40546, USA.
Reprod Domest Anim. 2013 Apr;48(2):297-304. doi: 10.1111/j.1439-0531.2012.02148.x. Epub 2012 Jul 16.
Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine expressed by a wide range of tissues, which has been implicated to be involved in reproduction. Relative abundance of MIF mRNA in conceptus and endometrial tissue was assessed using real-time RT-PCR. Western blot analysis and immunohistochemistry were used to detect MIF protein expression. MIF transcript abundance was lowest in conceptuses obtained 16 days after ovulation, while the remaining stages of conceptus development that were analysed showed relatively constant expression levels. Migration inhibitory factor expression localized to trophectoderm cells, while capsular material was void of MIF immunoreactivity. Throughout the oestrous cycle, no clear statistically significant cycle-dependent expression pattern could be observed. During early pregnancy, the highest mRNA transcript levels were detected 16 days after ovulation. Pregnancy status did not affect MIF mRNA expression. Using immunohistochemistry, MIF protein expression was primarily localized in luminal and glandular epithelial cells, while stromal cells displayed weaker immunoreactivity. Taken together, we suggest that MIF is part of the molecular repertoire that contributes to normal endometrial function. The detailed functional significance of MIF expression in equine endometrium and pre-implantation stages of conceptus development remains to be determined.
巨噬细胞移动抑制因子（MIF）是一种由多种组织表达的多效细胞因子，其与生殖过程有关。使用实时逆转录聚合酶链反应（RT-PCR）评估MIF mRNA在孕体和子宫内膜组织中的相对丰度。采用蛋白质免疫印迹分析和免疫组织化学检测MIF蛋白表达。排卵后16天获得的孕体中MIF转录本丰度最低，而分析的孕体发育其余阶段显示出相对恒定的表达水平。移动抑制因子表达定位于滋养外胚层细胞，而包膜物质缺乏MIF免疫反应性。在整个发情周期中，未观察到明显的统计学上显著的周期依赖性表达模式。在妊娠早期，排卵后16天检测到最高的mRNA转录水平。妊娠状态不影响MIF mRNA表达。使用免疫组织化学方法，MIF蛋白表达主要定位于腔上皮细胞和腺上皮细胞，而基质细胞显示较弱的免疫反应性。综上所述，我们认为MIF是有助于正常子宫内膜功能的分子组成部分。MIF在马子宫内膜和孕体植入前发育阶段表达的详细功能意义仍有待确定。
