用于评估适体-蛋白质相互作用的均相分析。

Homogeneous assay for evaluation of aptamer-protein interaction.

机构信息

Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary.

出版信息

Analyst. 2012 Sep 7;137(17):3929-31. doi: 10.1039/c2an35419e. Epub 2012 Jul 18.

DOI:10.1039/c2an35419e

PMID:22805733

Abstract

We introduce Amplified Luminescent Proximity Homogenous Assay (ALPHA) to assess the K(D) value of aptamer-protein complexes as demonstrated through the study of apple stem pitting virus coat protein-specific aptamers. This method can be used as a simple, cost-effective method for screening aptamer-target protein interactions during aptamer selection.

摘要

我们引入了扩增荧光邻近均相分析（ALPHA）来评估适体-蛋白质复合物的 K(D) 值，这在研究苹果茎痘病毒外壳蛋白特异性适体时得到了证明。该方法可作为一种简单、经济有效的方法，用于在适体筛选过程中筛选适体-靶蛋白相互作用。