Department of Microbial Biotechnology and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht road, Karaj, Iran.
World J Microbiol Biotechnol. 2012 Apr;28(4):1503-9. doi: 10.1007/s11274-011-0952-7. Epub 2011 Nov 19.
A biocontrol Streptomyces isolate (C) was tested for its plant growth promoting qualities under saline conditions. Exposure to elevated osmotic strengths up to 300 mM NaCl increased dry weight and cfu/ml significantly. The isolate C produced indolyl-3-acetic acid (IAA) into the medium in the amount of 2.4 μg/ml. The amount of auxin increased after adding salt and reached to 4.7 μg/ml in 300 mM NaCl. Biosynthesis of siderophore was detectable and increased in presence of NaCl. Streptomyces isolate C showed good solubilization of tricalcium phosphate in culture medium with 92 mg/l. Solubilization decreased in presence of NaCl. Soil treatment with isolate C increased the growth and development of wheat plant in normal and saline conditions. In this treatment there were significant increases in germination rate, percentage and uniformity, shoot length and dry weight compared to the control. Applying the bacterial inocula increased the concentration of N, P, Fe and Mn in wheat shoots grown in normal and saline soil, but had non-significant effect on other micro and macronutrients concentrations. Results of this study show that Streptomyces isolate C has potential to be utilized as biofertilizer in saline soils.
一种生防链霉菌（C）菌株在盐胁迫条件下被测试其促进植物生长的特性。暴露于高达 300mM NaCl 的渗透压强度下，显著增加了干重和 cfu/ml。菌株 C 在培养基中产生吲哚-3-乙酸（IAA），其含量为 2.4μg/ml。添加盐后，生长素的含量增加，在 300mM NaCl 中达到 4.7μg/ml。在存在 NaCl 的情况下，生物合成铁载体是可检测到的，并增加。链霉菌 C 菌株在含有 92mg/L 的三钙磷酸盐培养基中表现出良好的溶解能力。在存在 NaCl 的情况下，溶解能力下降。用菌株 C 处理土壤可促进小麦在正常和盐胁迫条件下的生长和发育。与对照相比，该处理显著提高了发芽率、发芽百分比和均匀度、株高和干重。应用细菌接种剂增加了在正常和盐土中生长的小麦地上部的氮、磷、铁和锰的浓度，但对其他微量和大量营养元素的浓度没有显著影响。本研究结果表明，链霉菌 C 菌株具有在盐渍土壤中作为生物肥料的应用潜力。
