Department of Chemical Engineering, Tuskegee University, 522E Luther Foster Hall, Tuskegee, AL 36088, USA.
World J Microbiol Biotechnol. 2012 May;28(5):2181-93. doi: 10.1007/s11274-012-1024-3. Epub 2012 Feb 24.
The microbial selection on an enhanced biological phosphorus removal (EBPR) system was investigated in a laboratory-scale sequencing batch reactor fed exclusively with glucose as the carbon source. Fluorescence In Situ Hybridization analysis was performed to target two polyphosphate accumulating organisms (PAOs) (i.e., Candidatus Accumulibacter phosphatis and Microlunatus phosphovorus) and two glycogen accumulating organisms (GAOs) (i.e., Candidatus Competibacter phosphatis and Micropruina glycogenica). The results show that glucose might not select for Candidatus Accumulibacter phosphatis. However, Microlunatus phosphovorus, Candidatus Competibacter phosphatis, and Micropruina glycogenica might be selected. The highest percent relative abundance (% RA) of Candidatus Accumulibacter phosphatis was about 42%; this occurred at the beginning of the experimental period when phosphorus removal was efficient. However, the % RA of these bacteria decreased, reaching below 4% at the end of the run. The maximum % RA of Microlunatus phosphovorus, Candidatus Competibacter phosphatis, and Micropruina glycogenica was about 21, 37, 17%, respectively. It appears that a higher glucose concentration might be detrimental for Microlunatus phosphovorus and Micropruina glycogenica. Results also indicate a dominance of GAOs over PAOs when EBPR systems are fed with glucose. It is possible that the GAOs outcompete the PAOs at low pH values; it has been reported that at low pH, GAOs use glycogen as the energy source to uptake glucose. As a result, P-removal deteriorated. Therefore, glucose is not a strong candidate as a carbon source to supplement EBPR systems that do not contain sufficient volatile fatty acids.
在实验室规模的序批式反应器中，仅以葡萄糖作为碳源，研究了对强化生物除磷 (EBPR) 系统的微生物选择。采用荧光原位杂交分析（FISH）技术，针对两种聚磷菌（PAOs）（即 Candidatus Accumulibacter phosphatis 和 Microlunatus phosphovorus）和两种糖原积累菌（GAOs）（即 Candidatus Competibacter phosphatis 和 Micropruina glycogenica）进行了目标分析。结果表明，葡萄糖可能不会选择 Candidatus Accumulibacter phosphatis。然而，Microlunatus phosphovorus、Candidatus Competibacter phosphatis 和 Micropruina glycogenica 可能会被选择。Candidatus Accumulibacter phosphatis 的相对丰度（% RA）最高约为 42%；这发生在实验初期，当时除磷效率较高。然而，这些细菌的 % RA 下降，在运行结束时降至 4%以下。Microlunatus phosphovorus、Candidatus Competibacter phosphatis 和 Micropruina glycogenica 的最大 % RA 分别约为 21%、37%和 17%。似乎较高的葡萄糖浓度可能对 Microlunatus phosphovorus 和 Micropruina glycogenica 不利。结果还表明，当 EBPR 系统以葡萄糖为碳源时，GAOs 比 PAOs 更占优势。当 pH 值较低时，GAOs 可能会利用糖原作为能量来源来吸收葡萄糖，从而使 GAOs 比 PAOs 更具优势，这已经得到了报道。因此，除磷恶化。因此，葡萄糖不是补充不含足够挥发性脂肪酸的 EBPR 系统的有力碳源。
