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田间豌豆(Pisum sativum L.)种皮中类胡萝卜素和多酚的基因型丰度。

Genotypic abundance of carotenoids and polyphenolics in the hull of field pea (Pisum sativum L.).

机构信息

Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada.

出版信息

J Sci Food Agric. 2013 Feb;93(3):463-70. doi: 10.1002/jsfa.5782. Epub 2012 Jul 17.

DOI:10.1002/jsfa.5782
PMID:22806437
Abstract

BACKGROUND

Consumption of pulse crops, including field pea, is considered effective for a healthy diet. Hulls (seed coats) play an important role for protection of the cotyledon and embryo, but also as mediating positive effects on health outcomes. The biochemical attributes of field pea hulls were thus assessed to determine the occurrence of specific phytochemicals and their genotypic variability.

RESULTS

Sequestered bioproducts in mature hulls predominantly consisted of trans-lutein and chlorophylls a and b. Trace amounts of other carotenoid and pheophytin metabolites were identified. In developing hulls, violaxanthin, neoxanthin, lutein, zeaxanthin, chlorophylls a and b and β-carotene were detected. Genotypic differences in the accumulation of lutein and chlorophylls a and b were observed over years and locations. Polyphenolics and hydroxybenzoic acids were detected in the 'dun' and 'maple' field pea types-the only genotypes to have pigmented hulls. Unextractable patches of condensed tannin influenced the visual uniformity of the maple and dun genotypes, CDC Rocket and CDC Dundurn.

CONCLUSIONS

Within the yellow and green market classes, carotenoid and chlorophyll accumulation was consistent. Green cotyledon varieties sequestered higher concentrations of lutein than the yellow cotyledon varieties. Maple and dun types were more variable, reflective of different selection criteria. The occurrence of flavonoid-related compounds was correlated only with pigmented seed coat genotypes. The dietary potential of the chlorophylls and carotenoids that accumulated in the hulls split from the green and yellow field pea types is discussed as a value-added prospect in food supplements.

摘要

背景

食用豆类作物,包括野豌豆,被认为对健康饮食有效。种皮(种子的外皮)在保护子叶和胚胎方面起着重要作用,但也对健康结果产生积极影响。因此,评估了野豌豆种皮的生化特性,以确定特定植物化学物质的存在及其基因型变异性。

结果

成熟种皮中蓄积的生物制品主要由反式叶黄素和叶绿素 a 和 b 组成。还鉴定出其他类胡萝卜素和脱镁叶绿素代谢物的痕量。在发育中的种皮中,检测到紫黄质、新黄质、叶黄素、玉米黄质、叶绿素 a 和 b 以及β-胡萝卜素。多年和不同地点观察到叶黄素和叶绿素 a 和 b 的积累存在基因型差异。在“dun”和“maple”野豌豆类型中检测到多酚和羟基苯甲酸——这是唯一具有有色种皮的基因型。未提取的浓缩单宁斑点影响了 maple 和 dun 基因型(CDC Rocket 和 CDC Dundurn)的视觉均匀性。

结论

在黄色和绿色市场类别中,类胡萝卜素和叶绿素的积累是一致的。绿色子叶品种积累的叶黄素浓度高于黄色子叶品种。maple 和 dun 类型更具变异性,反映了不同的选择标准。类黄酮相关化合物的出现仅与有色种皮基因型相关。从绿色和黄色野豌豆类型中分离出来的种皮中积累的叶绿素和类胡萝卜素的膳食潜力被讨论为食品补充剂的增值前景。

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