Microbial Technology, Central Institute of Medicinal and Aromatic Plants (CSIR), P.O. CIMAP, Lucknow, 226015, India.
World J Microbiol Biotechnol. 2012 Jan;28(1):323-33. doi: 10.1007/s11274-011-0823-2. Epub 2011 Jun 28.
The usefulness of vermicompost as a supporting media for growth of bioinoculants was evaluated for successful transfer of sufficient propagules of bioinoculants into the organic fields. The rooted plants after 50 days were pot and field tested for their growth and yield performances when transplanted along with rooting medium into pots/organic fields. The rooting medium, 50 days of inoculation, contained sufficient population of bioinoculants and arbuscular mycorrhizal (AM) fungi. Treatment with bioinoculants (except Trichoderma harzianum) substantially improved the root and shoot biomass of nursery raised rooted cuttings particularly in treatments containing Azotobacter chroococcum (150 and 91.67%, respectively), Glomus intraradices (117 and 91.67%, respectively) and Pseudomonas fluorescens (117 and 83%, respectively). The transplanted rooted plants in pots, over two harvests, yielded higher shoot biomass when rooting medium contained A. chroococcum (147%), G. intraradices (139%) and P. fluorescencs (139%). Although the treatments did not affect the content of essential oil, the quality of essential oil as measured by the content of patchouli alcohol improved with Glomus aggregatum (18%). Similar trends were observed in field trials with significantly higher biomass yield achieved with A. chroococcum (51%), G. intraradices (46%) and P. fluorescencs (17%) compared to control (un-inoculated) plots. Increased in herb yield was found to be related with increased nutrient uptake. The population of bioinoculants in the rhizosphere was observed to be considerably higher in plots receiving vermicompost enriched with bioinoculants. This technology can be a successful way of delivering sufficient propagules of bioinoculants along with vermicompost especially in organic fields.
评估了蚯蚓粪作为生物接种剂生长支持介质的有用性,以确保将足够数量的生物接种剂接种体成功转移到有机田地中。在 50 天后,将生根植物连同生根介质一起移植到盆中/有机田地中,对其生长和产量性能进行盆和田间测试。生根介质在 50 天的接种期内含有足够数量的生物接种剂和丛枝菌根真菌(AM)。除哈茨木霉(Trichoderma harzianum)外,生物接种剂处理显著提高了苗圃中生根插条的根和茎生物量,特别是在含有固氮菌(Azotobacter chroococcum)(分别为 150%和 91.67%)、摩西斗管囊霉(Glomus intraradices)(分别为 117%和 91.67%)和荧光假单胞菌(Pseudomonas fluorescens)(分别为 117%和 83%)的处理中。在两个收获期内,在盆中移植的生根植物在含有固氮菌(147%)、摩西斗管囊霉(139%)和荧光假单胞菌(139%)的生根介质中,地上部生物量产量更高。尽管处理没有影响精油的含量,但精油的质量,以对叶醇的含量来衡量,随着聚丛孢囊霉(Glomus aggregatum)(18%)的增加而提高。田间试验也观察到了类似的趋势,与对照(未接种)地块相比,固氮菌(51%)、摩西斗管囊霉(46%)和荧光假单胞菌(17%)的生物量产量显著提高。发现香草产量的增加与养分吸收的增加有关。在接受富含生物接种剂的蚯蚓粪处理的根际中,生物接种剂的种群数量明显更高。这项技术可以成功地将足够数量的生物接种剂与蚯蚓粪一起传递到有机田地中。