Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
World J Microbiol Biotechnol. 2012 Feb;28(2):749-53. doi: 10.1007/s11274-011-0835-y. Epub 2011 Jul 7.
The scale-up strategy for acarbose fermentation by Actinoplanes sp. A56 was explored in this paper. The results obtained in shake-flask cultivation demonstrated that the ratio of maltose and glucose had significant effects on the biosynthesis of acarbose, and the feeding medium containing 3:1 (mass ratio) of maltose and glucose was favorable for acarbose production. Then the correlation of the carbon source concentration with acarbose production was further investigated in 100-l fermenter, and the results showed that 7.5-8.0 g of total sugar/100 ml and 4.0-4.5 g of reducing sugar/100 ml were optimal for acarbose production. Based on the results in 100-l fermenter, an effective and simplified scale-up strategy was successfully established for acarbose fermentation in a 30-m(3) fermenter, by using total sugar and reducing sugar as the scale-up parameter. As a result, 4,327 mg of acarbose/l was obtained at 168 h of fermentation.
本文探讨了灰色链霉菌 A56 发酵阿卡波糖的放大策略。摇瓶培养的结果表明，麦芽糖和葡萄糖的比例对阿卡波糖的生物合成有显著影响，含 3:1（质量比）麦芽糖和葡萄糖的补料培养基有利于阿卡波糖的生产。然后在 100-L 发酵罐中进一步研究了碳源浓度与阿卡波糖生产的相关性，结果表明，100ml 中总糖为 7.5-8.0g 和还原糖为 4.0-4.5g 有利于阿卡波糖的生产。基于 100-L 发酵罐的结果，使用总糖和还原糖作为放大参数，成功地在 30m3 发酵罐中建立了阿卡波糖发酵的有效简化放大策略。结果，在 168 小时的发酵过程中获得了 4327mg/L 的阿卡波糖。
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