Institute for Biotechnology and Bioengineering (IBB), Centre for Biological Engineering, University of Minho, Braga, Portugal.
Macromol Biosci. 2012 Sep;12(9):1172-80. doi: 10.1002/mabi.201200075. Epub 2012 Jul 16.
The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.
在设计药物和疫苗递送系统时，必须仔细考虑与纳米药物细胞内化相关的机制。纳米药物的细胞命运和效果在很大程度上取决于细胞摄取途径。本文开发了一种自组装甘露聚糖纳米凝胶，作为抗原和佐剂递送的疫苗平台。研究发现，鼠源骨髓来源的巨噬细胞对甘露聚糖纳米凝胶的摄取依赖于时间、浓度和能量，涉及甘露糖受体介导的吞噬作用和网格蛋白介导的内吞作用。纳米凝胶也在细胞质中被可视化，表明其可以逃离内溶酶体。这些结果表明，甘露聚糖纳米凝胶是一种很有前途的多功能载体，可用于细胞内递送疫苗或治疗剂。
