Hamashima H, Nakano T, Tamura S, Arai T
Department of Microbiology, Showa College of Pharmaceutical Sciences, Tokyo.
Microbiol Immunol. 1990;34(8):703-8. doi: 10.1111/j.1348-0421.1990.tb01047.x.
An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains.
作为开发用于弧菌属基因操作的重组DNA技术工作的一部分，进行了用于弧菌属质粒介导转化的电穿孔程序。用3种载体质粒（pACYC184、pHSG398和pBR325）转化副溶血性弧菌、溶藻弧菌和非O-1群霍乱弧菌（9个不同菌株）。转化效率高度依赖三个参数：质粒DNA的浓度；电场强度；以及质粒DNA与受体菌株的组合。载体质粒上的耐药基因在弧菌菌株中表达。
