Kawashima Atsunari, Takayama Hitoshi, Kawamura Norihiko, Doi Noriteru, Sato Mototaka, Hatano Koji, Nagahara Akira, Uemura Motohide, Nakai Yasutomo, Nishimura Kensaku, Miyoshi Susumu, Kawano Kiyoshi, Nishimura Kazuo, Nonomura Norio, Tsujimura Akira
Department of Urology, Osaka University Graduate School of Medicine, Osaka.
Oncol Lett. 2012 Jul;4(1):15-21. doi: 10.3892/ol.2012.689. Epub 2012 Apr 23.
Neoadjuvant chemotherapy (NC) for bladder cancer has been reported to significantly improve the 5-year survival rate. The aim of the present study was to examine the roles of ERCC1 and Snail in determining the response to chemotherapy in bladder cancer treated with NC and radical cystectomy (RC). The expression of the Snail and ERCC1 proteins was determined by immunohistochemical staining of specimens obtained from 58 patients with bladder tumors treated with NC and RC. The correlation between clinical response and the expression of Snail and ERCC1 was investigated. Snail and ERCC1 were co-expressed in 24 (41.4%) of the 58 patients. A marked correlation was found between the expression of Snail and ERCC1 (P=0.001). The co-expression of Snail and ERCC1 was not able to predict pathological complete response (P=0.202). Results of the univariate analysis revealed that the co-expression of Snail and ERCC1 predicted shorter disease-free survival (DFS) and overall survival (OS) than the negative expression of Snail and/or ERCC1. Moreover, the co-expression of ERCC1 and Snail was the only predictive factor for both DFS (P=0.029) and OS (P=0.040). The expression of Snail was correlated with that of ERCC1 and the co-expression of Snail and ERCC1 was the only significant predictive factor of shorter DFS and OS in patients with bladder cancer treated with NC and RC.
据报道，膀胱癌新辅助化疗（NC）可显著提高5年生存率。本研究的目的是探讨ERCC1和Snail在接受NC和根治性膀胱切除术（RC）治疗的膀胱癌化疗反应中的作用。通过对58例接受NC和RC治疗的膀胱肿瘤患者的标本进行免疫组织化学染色，测定Snail和ERCC1蛋白的表达。研究临床反应与Snail和ERCC1表达之间的相关性。58例患者中有24例（41.4%）同时表达Snail和ERCC1。Snail和ERCC1的表达之间存在显著相关性（P=0.001）。Snail和ERCC1的共表达不能预测病理完全缓解（P=0.202）。单因素分析结果显示，与Snail和/或ERCC1阴性表达相比，Snail和ERCC1的共表达预示无病生存期（DFS）和总生存期（OS）更短。此外，ERCC1和Snail的共表达是DFS（P=0.029）和OS（P=0.040）的唯一预测因素。Snail的表达与ERCC1的表达相关，在接受NC和RC治疗的膀胱癌患者中，Snail和ERCC1的共表达是DFS和OS缩短的唯一显著预测因素。
