Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
J Gynecol Oncol. 2012 Jul;23(3):190-6. doi: 10.3802/jgo.2012.23.3.190. Epub 2012 Jul 2.
Epidemiological studies suggest that selenium protects against the development of several cancers. Selenium (sodium selenite) has been reported to interfere with cell growth and proliferation, and to induce cell death. In this study, we tested whether selenium could have growth-inhibiting effect in ovarian cancer cells and an orthotopic animal model.
Cell growth in selenium-treated cells was determined in human ovarian cancer cells, A2780, HeyA8, and SKOV3ip1 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay. Animal experiment of selenium with paclitaxel was performed using SKOV3ip1 cells in nude mice to evaluate their inhibiting effect for tumor growth. In addition, another animal experiment of paclitaxel with or without selenium was performed to assess the effect of survival and food intake in mice.
The in vitro growth of selenium-treated cells was significantly decreased dose-dependently in A2780, HeyA8, and SKOV3ip1 cells. Therapy experiment in mice was started 1 week after injection of the SKOV3ip1 cells. Treatment with selenium (1.5 mg/kg, 3 times/week) and paclitaxel injection showed no addictive effect of the inhibition of tumor growth. However, combination of selenium and paclitaxel showed the slightly increased food intake compared with paclitaxel alone.
Although selenium has growth-inhibiting effect in ovarian carcinoma cells in vitro, there is no additive effect on tumor growth in mice treated with combination of paclitaxel and selenium. However, food intake is slightly higher in selenium-treated mice during chemotherapy.
流行病学研究表明，硒可预防多种癌症的发生。有报道称，硒（亚硒酸钠）可干扰细胞生长和增殖，并诱导细胞死亡。本研究旨在检测硒对卵巢癌细胞及其原位动物模型是否具有生长抑制作用。
采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法检测人卵巢癌细胞 A2780、HeyA8 和 SKOV3ip1 中经硒处理后的细胞生长情况。采用 SKOV3ip1 细胞建立裸鼠原位动物模型，进行硒联合紫杉醇的动物实验，以评估其对肿瘤生长的抑制作用。此外，还进行了紫杉醇联合或不联合硒的另一项动物实验，以评估硒对小鼠生存和食物摄入的影响。
A2780、HeyA8 和 SKOV3ip1 细胞中，硒处理细胞的体外生长明显呈剂量依赖性下降。SKOV3ip1 细胞注射后 1 周开始进行治疗实验。每周 3 次给予 1.5mg/kg 硒和紫杉醇注射，并未显示出对肿瘤生长抑制的附加作用。然而，与紫杉醇单独治疗相比，硒联合紫杉醇治疗组的食物摄入量略有增加。
尽管硒对卵巢癌细胞具有体外生长抑制作用，但在联合紫杉醇治疗的小鼠中，对肿瘤生长没有附加作用。然而，在化疗期间，接受硒治疗的小鼠的食物摄入量略有增加。
