Meĭchik N R, Popova N I, Nikolaeva Iu I, Ermakov I P, Kamnev A N
Prikl Biokhim Mikrobiol. 2011 Mar-Apr;47(2):194-200.
Research into ion-exchange properties of cell walls isolated from thallus of red seaweed Phyllophora crispa was carried out. Ion-exchange capacity and the swelling coefficient of the red alga cell walls were estimated at various pH values (from 2 to 12) and at constant ionic strength of a solution (10 mM). It was established that behavior of cell walls as ion-exchangers is caused by the presence in their matrix of two types of cation-exchange groups and amino groups. The amount of the functional group of each type was estimated, and the corresponding values of pK(a) were calculated. It can be assumed that ionogenic groups with pK(a) -5 are carboxyl groups of uronic acids, and ionogenic groups with pK(a) -7.5 are carboxyl groups of the proteins. Intervals of pH in which cation-exchange groups are ionized and can take part in exchange reactions with cations in the environment are defined. It was found that protein was a major component of cell wall polymeric matrix because its content was 36%.
对从红藻皱波角叉菜叶状体中分离出的细胞壁的离子交换特性进行了研究。在不同pH值（2至12）和溶液恒定离子强度（10 mM）下，估算了红藻细胞壁的离子交换容量和膨胀系数。结果表明，细胞壁作为离子交换剂的行为是由其基质中存在的两种类型的阳离子交换基团和氨基引起的。估算了每种类型官能团的数量，并计算了相应的pK(a)值。可以假定，pK(a)为-5的离子ogenic基团是糖醛酸的羧基，而pK(a)为-7.5的离子ogenic基团是蛋白质的羧基。确定了阳离子交换基团发生电离并能参与与环境中阳离子交换反应的pH区间。研究发现，蛋白质是细胞壁聚合物基质的主要成分，因为其含量为36%。
