Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences No. 427, Harbin 150001, China.
Vet J. 2013 Feb;195(2):244-7. doi: 10.1016/j.tvjl.2012.06.005. Epub 2012 Jul 17.
Mycobacterium tuberculosis heat shock protein 70 (HSP70) and the peptide binding C-terminal portion of HSP70 (amino acids 359-610; HSP70c) exert an adjuvant effect when used in vaccines. To enhance the immunogenicity of a DNA vaccine against porcine circovirus type 2 (PCV2), recombinant plasmids encoding the PCV2 ORF2 (capsid) gene fused to full length hsp70 (pCA-TCH) or truncated C-terminal hsp70c (pCA-TCHc) were constructed. Immunisation of mice with pCA-TCHc induced higher serum immunoglobulin G antibody levels, stronger T helper 1 immune responses and lower PCV2 viral titres following challenge than immunisation with pCA-TCH or Cap plasmids only.
结核分枝杆菌热休克蛋白 70（HSP70）和 HSP70 的肽结合 C 端部分（氨基酸 359-610；HSP70c）在疫苗中具有佐剂作用。为了增强针对猪圆环病毒 2 型（PCV2）的 DNA 疫苗的免疫原性，构建了编码 PCV2 ORF2（衣壳）基因与全长 hsp70（pCA-TCH）或截短的 C 端 hsp70c（pCA-TCHc）融合的重组质粒。与仅用 pCA-TCH 或 Cap 质粒免疫相比，用 pCA-TCHc 免疫小鼠可诱导更高的血清 IgG 抗体水平、更强的辅助性 T 细胞 1 型免疫反应和更低的 PCV2 病毒载量。
