Pacific Biosciences Research Center, University of Hawaii, Honolulu, HI 96822, USA.
Gen Comp Endocrinol. 2012 Sep 15;178(3):459-62. doi: 10.1016/j.ygcen.2012.07.005. Epub 2012 Jul 15.
The role of the small-conductance, calcium-activated SK potassium channel in regulating pancreatic β cell function remains controversial with conflicting pharmacological results. In this study, we used current clamp recordings to further characterize the function of SK channels in INS-1 cell line. We compared afterhyperpolarization potential (AHP) responses of SK1-downregulated cells with those of control INS-1 cells. They were tested with and without the presence of glucose. We found that cells in which SK1 channel subunit expression had been downregulated exhibited AHPs in the presence of 20mM glucose while control INS-1 cells had AHPs only in the absence of glucose. Our findings show that the glucose-dependence of the AHP in the rat INS-1 cell line depends only on SK1 channel subunit expression.
小电导钙激活 SK 钾通道在调节胰腺β细胞功能中的作用仍存在争议，其药理学结果相互矛盾。在这项研究中，我们使用电流钳记录进一步研究 SK 通道在 INS-1 细胞系中的功能。我们比较了 SK1 下调细胞与对照 INS-1 细胞的后超极化电位 (AHP) 反应。在有或没有葡萄糖的情况下对它们进行了测试。我们发现，下调 SK1 通道亚基表达的细胞在存在 20mM 葡萄糖的情况下表现出 AHP，而对照 INS-1 细胞仅在没有葡萄糖的情况下表现出 AHP。我们的研究结果表明，大鼠 INS-1 细胞系 AHP 的葡萄糖依赖性仅取决于 SK1 通道亚基的表达。
