Laboratory of Microbial Technology, Division of Applied Molecular Microbiology and Biomass Chemistry, Department of Bioscience and Biotechnology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
J Biosci Bioeng. 2012 Nov;114(5):526-30. doi: 10.1016/j.jbiosc.2012.06.001. Epub 2012 Jul 17.
We previously reported a butanol production process with pH-stat continuous feeding of dl-lactic acid and glucose as the co-substrate (Oshiro et al., Appl. Microbiol. Biotechnol., 87, 1177-1185, 2010). To accomplish butanol production from completely inedible substrates, in this study, we investigated acetone-butanol-ethanol (ABE) fermentation of Clostridium saccharoperbutylacetonicum N1-4 with lactic acid by using pentose as the co-substrate. Examination for optimum co-substrate indicated that arabinose was superior to glucose and xylose for ABE fermentation. Actually batch culture with lactic acid and arabinose without pH control exhibited higher butanol production (7.11 g/l) and lactic acid consumption (2.02 g) than those (6.62 g/l and 1.45 g, respectively) with glucose. Fed-batch culture without pH control increased these values to 12.08 g/l and 15.60 g/l butanol production, and to 3.83 g and 5.91 g lactic acid consumption by feeding the substrate once and twice, respectively. Finally, the result of gas chromatography-mass spectroscopy analysis using [1,2,3-(13)C(3)]-lactic acid indicated that lactic acid was converted to butanol with the efficiency of 51.9%. Thus, we established a novel high butanol production from lactic acid using arabinose as the co-substrate in simple fed-batch culture.
我们之前报道过一种丁醇生产工艺，采用 pH -stat 连续进料 dl-乳酸和葡萄糖作为共底物（Oshiro 等人，应用微生物学与生物技术，87，1177-1185，2010）。为了从完全不可食用的底物中生产丁醇，在这项研究中，我们研究了 Clostridium saccharoperbutylacetonicum N1-4 用戊糖作为共底物进行丙酮丁醇乙醇（ABE）发酵。最佳共底物的研究表明，阿拉伯糖比葡萄糖和木糖更适合 ABE 发酵。实际上，无 pH 控制的乳酸和阿拉伯糖分批培养表现出更高的丁醇产量（7.11 g/l）和乳酸消耗（2.02 g），而葡萄糖分别为 6.62 g/l 和 1.45 g。无 pH 控制的分批补料培养通过一次性和两次性进料，将这些值分别提高到 12.08 g/l 和 15.60 g/l 的丁醇产量，以及 3.83 g 和 5.91 g 的乳酸消耗。最后，使用 [1,2,3-(13)C(3)]-乳酸的气相色谱-质谱分析结果表明，乳酸的转化率为 51.9%。因此，我们建立了一种新型的高效丁醇生产工艺，使用阿拉伯糖作为共底物，在简单的补料分批培养中进行。
