Fei Ling-Na, Wang Qi-Zhao, Xu Rui-An
Institute of Molecular Medicine, Huaqiao University, Quanzhou 362021, China.
Yao Xue Xue Bao. 2012 May;47(5):573-9.
RNA interference (RNAi) has been proved as a novel approach for gene therapy. However, RNAi mono-therapy only aims at single gene, it therefore may ultimately fail to cure cancers caused by polygene variation. To overcome the deficiency of RNAi mono-therapy, "combinatorial RNA interference" (coRNAi) was put forward as a new strategy. By co-expressing the inducers of RNAi triggering single or multiple targets directly and other RNA- or protein-based silencers, coRNAi keeps target genes silent, prevents carcinogenic progression and induces apoptosis of tumor cells. This paper mainly reviews the major strategies of coRNAi and their applications in cancer gene therapy.
RNA干扰（RNAi）已被证明是一种新型的基因治疗方法。然而，RNAi单一疗法仅针对单个基因，因此最终可能无法治愈由多基因变异引起的癌症。为了克服RNAi单一疗法的不足，“组合RNA干扰”（coRNAi）作为一种新策略被提出。通过直接共表达触发单个或多个靶点的RNAi诱导剂以及其他基于RNA或蛋白质的沉默子，coRNAi可使靶基因保持沉默，阻止致癌进程并诱导肿瘤细胞凋亡。本文主要综述了coRNAi的主要策略及其在癌症基因治疗中的应用。
