Li Qiuying, Zhang Qiaozhi, Han Lulu, Yuan Zhe, Tan Jian, Du Bin, Zou Xiangyang, Hou Lin
College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Biol Bull. 2012 Jun;222(3):182-91. doi: 10.1086/BBLv222n3p182.
Nuclear protein 1 (NURP1) is a stress-related protein and closely related to diapause in the development of Artemia. In the present paper, the full-length 568-bp cDNA sequence of the nurp1 homolog of Artemia sinica (As-nurp1) was isolated by RACE technology for the first time. The putative As-nurp1 protein consists of 66 amino acids with a basic helix-loop-helix (bHLH) motif and a bipartite nuclear localization signal (NLS). Multiple sequence alignments revealed that the putative As-nurp1 protein sequence was relatively conserved across species, especially in the bHLH domain. The expression of As-nurp1 is widely distributed during A. sinica development. This is followed by a dramatic downregulation after diapause and is newly upregulated from the larval nauplius stage. Furthermore, As-nurp1 transcripts are highly upregulated under conditions of high salinity and low temperature. These findings suggest that As-nurp1 is stress-related and may act as a protective factor in embryonic development.
核蛋白1（NURP1）是一种与应激相关的蛋白，与卤虫发育过程中的滞育密切相关。在本论文中，首次通过RACE技术分离出中华卤虫（As-nurp1）nurp1同源物的全长568bp cDNA序列。推测的As-nurp1蛋白由66个氨基酸组成，具有一个碱性螺旋-环-螺旋（bHLH）基序和一个双分型核定位信号（NLS）。多序列比对显示，推测的As-nurp1蛋白序列在物种间相对保守，尤其是在bHLH结构域。As-nurp1的表达在中华卤虫发育过程中广泛分布。随后在滞育后急剧下调，并从幼虫无节幼体阶段重新上调。此外，As-nurp1转录本在高盐度和低温条件下高度上调。这些发现表明As-nurp1与应激相关，可能在胚胎发育中作为一种保护因子发挥作用。
