液相色谱法分析婴儿配方奶粉和成人/儿童营养配方奶粉中的5'-单核苷酸：2011年首次行动。20.

Analysis of 5'-mononucleotides in infant formula and adult/pediatric nutritional formula by liquid chromatography: First Action 2011.20.

作者信息

Gill Brendon D, Indyk Harvey E, Kumar Maureen C, Sievwright Nathan K, Manley-Harris Merilyn, Dowell Dawn

机构信息

Fonterra Co-operative Group Ltd, New Zealand.

出版信息

J AOAC Int. 2012 May-Jun;95(3):599-602. doi: 10.5740/jaoacint.cs2011_20.

DOI:10.5740/jaoacint.cs2011_20

PMID:22816249

Abstract

A method for the routine determination of 5'-mononucleotides (uridine 5'-monophosphate, inosine 5'-monophosphate, adenosine 5'-monophosphate, guanosine 5'-monophosphate, and cytidine 5'-monophosphate) in infant formula and adult nutritionals is described. After sample dissolution and addition of internal standard, potential interferences were removed by anion-exchange SPE followed by HPLC-UV analysis. Single-laboratory validation performance parameters include recovery (92-101%) and repeatability (1.0-2.3% RSD). The method was approved for Official First Action status by an AOAC expert review panel.

摘要

描述了一种用于常规测定婴儿配方奶粉和成人营养产品中5'-单核苷酸（尿苷5'-单磷酸、肌苷5'-单磷酸、腺苷5'-单磷酸、鸟苷5'-单磷酸和胞苷5'-单磷酸）的方法。样品溶解并加入内标后，通过阴离子交换固相萃取去除潜在干扰物，然后进行高效液相色谱-紫外分析。单实验室验证性能参数包括回收率（92-101%）和重复性（相对标准偏差为1.0-2.3%）。该方法经AOAC专家评审小组批准为官方首次采用状态。