比较胎盘血显微镜检查和 ICT HRP2 快速诊断检测法在诊断胎盘疟疾中的应用。

Comparison of placental blood microscopy and the ICT HRP2 rapid diagnostic test to detect placental malaria.

机构信息

Barcelona Centre for International Health Research (CRESIB), Hospital Clínic-Universitat de Barcelona, Barcelona, Spain.

出版信息

Trans R Soc Trop Med Hyg. 2012 Sep;106(9):573-5. doi: 10.1016/j.trstmh.2012.05.001. Epub 2012 Jul 20.

DOI:10.1016/j.trstmh.2012.05.001

PMID:22818744

Abstract

Monitoring interventions to prevent malaria in pregnancy requires sensitive detection of placental infection. Rapid diagnostic tests (RDTs) are good candidates, but little information is available on their sensitivity on placental blood. We have evaluated the agreement (kappa coefficient) between microscopy and a Plasmodium falciparum histidine-rich protein 2 (HRP2)-based immuno-chromatographic test (ICT) on placental blood from 1151 women at delivery. Prevalences of placental infection by microscopy and RDT were 5.1% and 5.0%, respectively, showing 82.9% agreement (p<0.0001). Discordances were found at low parasitemias (<500 parasites/μL) or negative microscopy. The results suggest that the HRP2-RDTs from ICT diagnostics is a good alternative to microscopy for diagnosing placental malaria at delivery.

摘要

监测预防妊娠疟疾的干预措施需要敏感地检测胎盘感染。快速诊断检测（RDT）是很好的候选方法，但关于其在胎盘血中的敏感性的信息很少。我们评估了在分娩时的 1151 名妇女的胎盘血中显微镜检查和基于恶性疟原虫 HRP2 的免疫层析检测（ICT）之间的一致性（kappa 系数）。通过显微镜检查和 RDT 检测到的胎盘感染的患病率分别为 5.1%和 5.0%，显示出 82.9%的一致性（p<0.0001）。在低寄生虫血症（<500 个寄生虫/μL）或阴性显微镜检查时发现了差异。结果表明，ICT 诊断的 HRP2-RDT 是诊断分娩时胎盘疟疾的显微镜检查的良好替代方法。