NADPH 氧化酶在人胰岛中的表达。

Expression of NADPH oxidase in human pancreatic islets.

机构信息

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.

出版信息

Life Sci. 2012 Sep 17;91(7-8):244-9. doi: 10.1016/j.lfs.2012.07.004. Epub 2012 Jul 17.

DOI:10.1016/j.lfs.2012.07.004

PMID:22820165

Abstract

AIMS

NADPH oxidase (NOX) is a known source of superoxide anions in phagocytic and non-phagocytic cells. In this study, the presence of this enzyme in human pancreatic islets and the importance of NADPH oxidase in human β-cell function were investigated.

MAIN METHODS AND KEY FINDINGS

In isolated human pancreatic islets, the expression of NADPH oxidase components was evidenced by real-time PCR (p22(PHOX), p47(PHOX) and p67(PHOX)), Western blotting (p47(PHOX) and p67(PHOX)) and immunohistochemistry (p47(PHOX), p67(PHOX) and gp91(PHOX)). Immunohistochemistry experiments showed co-localization of p47(PHOX), p67(PHOX) and gp91(PHOX) (isoform 2 of NADPH oxidase-NOX2) with insulin secreting cells. Inhibition of NADPH oxidase activity impaired glucose metabolism and glucose-stimulated insulin secretion.

SIGNIFICANCE

These findings demonstrate the presence of the main intrinsic components of NADPH oxidase comprising the NOX2 isoform in human pancreatic islets, whose activity also contributes to human β-cell function.

摘要

目的

NADPH 氧化酶（NOX）是吞噬细胞和非吞噬细胞中超氧阴离子的已知来源。在这项研究中，研究了这种酶在人胰岛中的存在以及 NADPH 氧化酶在人β细胞功能中的重要性。

主要方法和关键发现

在分离的人胰岛中，通过实时 PCR（p22（PHOX）、p47（PHOX）和 p67（PHOX））、Western 印迹（p47（PHOX）和 p67（PHOX））和免疫组织化学（p47（PHOX）、p67（PHOX）和 gp91（PHOX））证实了 NADPH 氧化酶成分的表达。免疫组织化学实验显示 p47（PHOX）、p67（PHOX）和 gp91（PHOX）（NADPH 氧化酶-NOX2 的同工型 2）与胰岛素分泌细胞的共定位。NADPH 氧化酶活性的抑制损害了葡萄糖代谢和葡萄糖刺激的胰岛素分泌。