Yang Feng-Qing, Yang Jing, Zhang Xue-Mei, Xu Pan, Xia Zhi-Ning
College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China.
J Chromatogr Sci. 2013 Feb;51(2):155-60. doi: 10.1093/chromsci/bms120. Epub 2012 Jul 23.
Volatile chemicals are a group of very important compounds in natural products. Curcuma rhizome, which contains many bioactive volatile compounds, is a traditional Chinese medicine that has long been used for the treatment of several diseases. In the present study, a microemulsion electrokinetic chromatography (MEEKC) method was developed for the analysis of four volatile components in Curcuma rhizome, including germacrone, furanodiene, curcumenol and curdione. Experimental parameters, including the pH, type and concentrations of background electrolyte, and microemulsion compositions (type and concentrations of surfactant, co-surfactant and oil phase) were intensively investigated. Finally, the primary compounds in the methanol extract of Curcuma rhizome were separated within 30 min using a running buffer composed of 2.31% w/v (80 mmol/L) sodium dodecyl sulfate (SDS), 0.91% w/v (80 mmol/L) 1-octane, 6.95% w/v (937.5 mmol/L) 1-butanol and 1.88% w/v (312.5 mmol/L) propanol in a 5-mM borate buffer (pH 8.1). The contents of the four investigated compounds were determined in the rhizome from C. phaeocaulis. The results showed that the developed MEEKC method provided an alternative tool for the analysis of volatile components, especially those of heat-sensitive compounds from natural products.
挥发性化学物质是天然产物中一类非常重要的化合物。姜黄根茎含有许多具有生物活性的挥发性化合物，是一种长期用于治疗多种疾病的传统中药。在本研究中，开发了一种微乳液电动色谱（MEEKC）方法，用于分析姜黄根茎中的四种挥发性成分，包括莪术酮、莪术二烯、莪术醇和莪术二酮。对实验参数进行了深入研究，包括背景电解质的pH值、类型和浓度，以及微乳液组成（表面活性剂、助表面活性剂和油相的类型和浓度）。最后，使用由2.31% w/v（80 mmol/L）十二烷基硫酸钠（SDS）、0.91% w/v（80 mmol/L）正辛烷、6.95% w/v（937.5 mmol/L）正丁醇和1.88% w/v（312.5 mmol/L）丙醇组成的运行缓冲液，在5 mM硼酸盐缓冲液（pH 8.1）中，在30分钟内分离了姜黄根茎甲醇提取物中的主要化合物。测定了蓬莪术根茎中四种被研究化合物的含量。结果表明，所开发的MEEKC方法为挥发性成分的分析提供了一种替代工具，特别是用于分析天然产物中热敏性化合物的挥发性成分。
