Arvidsson U, Schalling M, Cullheim S, Ulfhake B, Terenius L, Verhofstad A, Hökfelt T
Department of Anatomy, Karolinska Institutet, Stockholm, Sweden.
Brain Res. 1990 Nov 5;532(1-2):47-57. doi: 10.1016/0006-8993(90)91740-8.
By the use of the indirect immunofluorescence and in situ hybridization techniques, the distribution of calcitonin gene-related peptide (CGRP)-like immunoreactivity (LI) and CGRP mRNA was studied in the spinal cord as well as in the midline raphe nuclei and the hypoglossal nucleus in the medulla oblongata of the monkey (Macaca fascicularis). In the spinal cord only a few large neurons in the motor nucleus contained CGRP-LI, while a majority of the neurons in the hypoglossal nucleus contained CGRP-LI. A relatively dense innervation by CGRP-immunoreactive (IR) fibers was also seen close to cell bodies and proximal dendrites of large neurons in the motor nucleus, especially in its ventral part. 5-Hydroxytryptamine (5-HT)-, substance P- and thyrotropin-releasing hormone (TRH)-IR varicosities were also observed in a similar position around large neurons in the motor nucleus. Double labeling disclosed that the majority of CGRP-IR axon terminals also contained 5-HT-LI. Expression of CGRP mRNA was found in neurons in the medullary midline raphe nuclei and in large neurons in the motor nucleus at the cervical spinal cord level. In adjacent sections of the medulla oblongata, CGRP-labeled neurons in the midline raphe nuclei also expressed preprotachykinin mRNA. The present results show that CGRP- and 5-HT-LI coexist in fibers within the motor nucleus of the monkey spinal cord and that this coexistence is probably due to the presence of CGRP in the descending bulbospinal, serotonergic pathway.
运用间接免疫荧光和原位杂交技术，研究了降钙素基因相关肽（CGRP）样免疫反应性（LI）和CGRP mRNA在恒河猴（食蟹猴）延髓的脊髓、中缝核和舌下神经核中的分布情况。在脊髓中，运动核内只有少数大神经元含有CGRP-LI，而舌下神经核中的大多数神经元含有CGRP-LI。在运动核大神经元的胞体和近端树突附近，尤其是其腹侧部分，也可见到CGRP免疫反应性（IR）纤维的相对密集支配。在运动核大神经元周围的类似位置，还观察到5-羟色胺（5-HT）、P物质和促甲状腺激素释放激素（TRH）免疫反应性膨体。双重标记显示，大多数CGRP-IR轴突终末也含有5-HT-LI。在延髓中缝核的神经元以及颈髓水平运动核的大神经元中发现了CGRP mRNA的表达。在延髓相邻切片中，中缝核中CGRP标记的神经元也表达前速激肽原mRNA。目前的结果表明，CGRP和5-HT-LI在猴脊髓运动核内的纤维中共存，这种共存可能是由于在下行的延髓脊髓5-羟色胺能通路中存在CGRP。
