通过杂交遗传-化学分子转子标签的荧光寿命成像，绘制活细胞中蛋白质特异性微环境图谱。
Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.
机构信息
Department of Chemistry, Columbia University, 3000 Broadway and 119th Street, New York, NY, USA.
出版信息
Chem Commun (Camb). 2012 Sep 7;48(69):8694-6. doi: 10.1039/c2cc33133k. Epub 2012 Jul 24.
Abstract
The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.
摘要
特定蛋白质在活细胞内所经历的微观粘度和分子拥挤，可以调节它们的动力学和功能。利用新兴的 TMP-tag 技术，我们设计、合成并应用了一种杂交遗传-化学分子转子探针，其荧光寿命可以报告活细胞中特定蛋白质的微环境。
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