ENT Department, Medical School, Goethe University, Frankfurt am Main, Germany.
Oncol Rep. 2012 Sep;28(3):785-90. doi: 10.3892/or.2012.1903. Epub 2012 Jul 6.
One challenge of squamous cell carcinoma of the head and neck (SCCHN) chemotherapy is a small percentage of tumor cells that arrest in the G0 phase of the cell cycle and are thus not affected by chemotherapy. This could be one reason for tumor recurrence at a later date. The recruitment of these G0-arresting cells into the active cell cycle and thus, proliferation, may increase the efficacy of chemotherapeutic agents. The aim of this study was to investigate whether stimulation with recombinant epidermal growth factor (EGF) or serotonin leads to an increased tumor cell proliferation in xenografts. Detroit 562 cells were injected into NMRI-Foxn1nu mice. Treatment was performed with 15 µg murine or human EGF, or 200 µg serotonin. The control mice were treated with Lactated Ringer's solution (5 mice/group). Tumor size was measured on days 4, 8 and 12 after tumor cell injection. The EGF stimulated mice showed a significantly higher tumor growth compared to the serotonin-stimulated mice and the untreated controls. In the present study, we show that it is possible to stimulate tumor cells in xenografts by EGF and thus, enhance cell proliferation, resulting in a higher tumor growth compared to the untreated control group. In our future investigations, we plan to include a higher number of mice, an adjustment of the EGF dosage and cell subanalysis, considering the heterogeneity of SCCHN tumors.
头颈部鳞状细胞癌（SCCHN）化疗的一个挑战是，一小部分肿瘤细胞停留在细胞周期的 G0 期，因此不受化疗的影响。这可能是肿瘤日后复发的一个原因。招募这些 G0 期停滞的细胞进入活跃的细胞周期并增殖，可能会增加化疗药物的疗效。本研究旨在探讨表皮生长因子（EGF）或 5-羟色胺刺激是否会导致异种移植物中的肿瘤细胞增殖增加。将 Detroit 562 细胞注射到 NMRI-Foxn1nu 小鼠中。用 15μg 鼠或人 EGF 或 200μg 5-羟色胺进行治疗。对照组小鼠用乳酸林格氏液（每组 5 只）处理。在肿瘤细胞注射后第 4、8 和 12 天测量肿瘤大小。与 5-羟色胺刺激组和未处理对照组相比，EGF 刺激组的小鼠肿瘤生长明显更快。在本研究中，我们证明通过 EGF 刺激异种移植物中的肿瘤细胞，从而增强细胞增殖，与未处理对照组相比，肿瘤生长更高。在我们未来的研究中，我们计划包括更多的小鼠、调整 EGF 剂量和细胞亚分析，考虑到 SCCHN 肿瘤的异质性。
